Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33042 | 5' | -50 | NC_007497.1 | + | 26737 | 0.66 | 0.909015 |
Target: 5'- gCUCGGCGGUGCcUGUaccgAUGGCG-GCAc -3' miRNA: 3'- -GAGUCGUCAUGuGCG----UAUUGCaCGUc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 2428 | 0.66 | 0.894536 |
Target: 5'- gCUUAGCAGUGCcgguGCGCcgcugGGCGUucgcGCGGc -3' miRNA: 3'- -GAGUCGUCAUG----UGCGua---UUGCA----CGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 8520 | 0.66 | 0.886845 |
Target: 5'- cCUUGGCAGcuUugGCuaucccguucgGCGUGACGUGCGa -3' miRNA: 3'- -GAGUCGUC--AugUG-----------CGUAUUGCACGUc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 28866 | 0.66 | 0.88606 |
Target: 5'- gCUCGGCGGUGuCGCGCA-AAUGUugagcccGCAu -3' miRNA: 3'- -GAGUCGUCAU-GUGCGUaUUGCA-------CGUc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 9541 | 0.67 | 0.870589 |
Target: 5'- aUCGGgAGUACACGCG-GACG-GCc- -3' miRNA: 3'- gAGUCgUCAUGUGCGUaUUGCaCGuc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 32588 | 0.67 | 0.841357 |
Target: 5'- uCUCGGCGG-ACugGCGggcggccugucggaUGGCGUcGCGGc -3' miRNA: 3'- -GAGUCGUCaUGugCGU--------------AUUGCA-CGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 36792 | 0.68 | 0.825221 |
Target: 5'- --aAGCaAGUACAgcCGCGUGA-GUGCAGa -3' miRNA: 3'- gagUCG-UCAUGU--GCGUAUUgCACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 28185 | 0.68 | 0.812431 |
Target: 5'- aCUCGGUAGccgaACACGCcgGACGacgggucacucuucUGCAGc -3' miRNA: 3'- -GAGUCGUCa---UGUGCGuaUUGC--------------ACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 33516 | 0.68 | 0.805393 |
Target: 5'- -gCAGCAGcACGCGCc---CGUGCAa -3' miRNA: 3'- gaGUCGUCaUGUGCGuauuGCACGUc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 29528 | 0.7 | 0.707555 |
Target: 5'- -aUAGCAGUcgaGCACGC----CGUGCAGa -3' miRNA: 3'- gaGUCGUCA---UGUGCGuauuGCACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 39215 | 0.7 | 0.684543 |
Target: 5'- uUCAGCAGgccgccgccgACGCGCuucguCGUGUAGa -3' miRNA: 3'- gAGUCGUCa---------UGUGCGuauu-GCACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 25454 | 0.71 | 0.626249 |
Target: 5'- aUCGGCuGGUGCugGacggcaGUAGCGUGCGc -3' miRNA: 3'- gAGUCG-UCAUGugCg-----UAUUGCACGUc -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 32030 | 0.72 | 0.568082 |
Target: 5'- uUCGGCgaugGGUACACGCA--GCGcGCAGc -3' miRNA: 3'- gAGUCG----UCAUGUGCGUauUGCaCGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 32322 | 0.73 | 0.556584 |
Target: 5'- -gCAGCGGUGCugGUGcUGGCGgGCAGu -3' miRNA: 3'- gaGUCGUCAUGugCGU-AUUGCaCGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 2893 | 0.75 | 0.436306 |
Target: 5'- -gCAcGCGGUGCugGCGcAugGUGCGGa -3' miRNA: 3'- gaGU-CGUCAUGugCGUaUugCACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 26951 | 0.76 | 0.386756 |
Target: 5'- -gCAGCuGUAC-CGCGUcgucAGCGUGCAGg -3' miRNA: 3'- gaGUCGuCAUGuGCGUA----UUGCACGUC- -5' |
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33042 | 5' | -50 | NC_007497.1 | + | 12267 | 1.1 | 0.002127 |
Target: 5'- uCUCAGCAGUACACGCAUAACGUGCAGa -3' miRNA: 3'- -GAGUCGUCAUGUGCGUAUUGCACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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