Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33043 | 5' | -56.3 | NC_007497.1 | + | 4441 | 0.66 | 0.616486 |
Target: 5'- cCGCGG-CU--ACaaGAUGCGUAGCGAg -3' miRNA: 3'- -GCGCCaGAcgUGgaCUAUGCGUCGCU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 18777 | 0.66 | 0.605389 |
Target: 5'- gGCGGcgaagCUGCGCa-GAUACGcCGGCa- -3' miRNA: 3'- gCGCCa----GACGUGgaCUAUGC-GUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 35824 | 0.67 | 0.550431 |
Target: 5'- aGCGG-CUGCcgcguuGCUUgcgcGAUGCGCAGCa- -3' miRNA: 3'- gCGCCaGACG------UGGA----CUAUGCGUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 36716 | 0.67 | 0.539604 |
Target: 5'- uGCGcGUCUGCACUc---ACGCGGCu- -3' miRNA: 3'- gCGC-CAGACGUGGacuaUGCGUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 11239 | 0.67 | 0.539604 |
Target: 5'- uCGCGGUCgGgGCCaaUGAUGCG-AGCa- -3' miRNA: 3'- -GCGCCAGaCgUGG--ACUAUGCgUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 13534 | 0.67 | 0.539604 |
Target: 5'- gCGCGGaaggUCgaaCACCUGAUgcaagaccaGCGCAGCa- -3' miRNA: 3'- -GCGCC----AGac-GUGGACUA---------UGCGUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 25134 | 0.67 | 0.518178 |
Target: 5'- aGaCGGcUCUGCGCCaGAgACGCGGUa- -3' miRNA: 3'- gC-GCC-AGACGUGGaCUaUGCGUCGcu -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 39272 | 0.68 | 0.476413 |
Target: 5'- gGCGGcCUGCugaauccggaGCC-GAUGCGC-GCGAu -3' miRNA: 3'- gCGCCaGACG----------UGGaCUAUGCGuCGCU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 19057 | 0.68 | 0.446213 |
Target: 5'- aGCGGUaucgGCugCUGcgACGCGGUcaGAa -3' miRNA: 3'- gCGCCAga--CGugGACuaUGCGUCG--CU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 39035 | 0.69 | 0.436386 |
Target: 5'- aGCGGUCgGCGCaCUGG-AgGguGCGGa -3' miRNA: 3'- gCGCCAGaCGUG-GACUaUgCguCGCU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 23567 | 0.74 | 0.198025 |
Target: 5'- gCGCGGcggcgggcgccggcgUCgUGCACCUGAUcaGCGCGcGCGAg -3' miRNA: 3'- -GCGCC---------------AG-ACGUGGACUA--UGCGU-CGCU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 11380 | 0.77 | 0.13688 |
Target: 5'- aGUGGgg-GCGCCUGAagucgGCGCGGCGAc -3' miRNA: 3'- gCGCCagaCGUGGACUa----UGCGUCGCU- -5' |
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33043 | 5' | -56.3 | NC_007497.1 | + | 13552 | 1.09 | 0.000602 |
Target: 5'- uCGCGGUCUGCACCUGAUACGCAGCGAa -3' miRNA: 3'- -GCGCCAGACGUGGACUAUGCGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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