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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33044 | 5' | -51.1 | NC_007497.1 | + | 8542 | 0.66 | 0.829593 |
Target: 5'- -cGCCGCGCAaaaugcggcugaUCGUCggcugcggaacuccAGUGucGCGUGAa -3' miRNA: 3'- gaCGGUGCGU------------AGUAG--------------UCAUu-CGCACU- -5' |
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33044 | 5' | -51.1 | NC_007497.1 | + | 15748 | 0.67 | 0.792927 |
Target: 5'- uCU-CCACcgGCGUCAUCGGUGGGUGcucgGAg -3' miRNA: 3'- -GAcGGUG--CGUAGUAGUCAUUCGCa---CU- -5' |
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33044 | 5' | -51.1 | NC_007497.1 | + | 16695 | 0.68 | 0.739456 |
Target: 5'- uUGCCACGCuUCuUCcGUGAaCGUGAu -3' miRNA: 3'- gACGGUGCGuAGuAGuCAUUcGCACU- -5' |
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33044 | 5' | -51.1 | NC_007497.1 | + | 19070 | 0.7 | 0.613551 |
Target: 5'- gCUGCgACGCG--GUCAGaagugGAGCGUGGu -3' miRNA: 3'- -GACGgUGCGUagUAGUCa----UUCGCACU- -5' |
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33044 | 5' | -51.1 | NC_007497.1 | + | 14334 | 1.09 | 0.001732 |
Target: 5'- gCUGCCACGCAUCAUCAGUAAGCGUGAu -3' miRNA: 3'- -GACGGUGCGUAGUAGUCAUUCGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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