Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33045 | 3' | -53.9 | NC_007497.1 | + | 8682 | 0.7 | 0.44886 |
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Target: 5'- cGCGUc-GGCCGCccGGUCGUgugGCUGCg -3' miRNA: 3'- uCGUAucUCGGCG--CCAGUAaa-CGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 4263 | 0.71 | 0.42883 |
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Target: 5'- cGCAUGGcgccggaauuGGCgGCGaaucGUCcgUUGCCGCa -3' miRNA: 3'- uCGUAUC----------UCGgCGC----CAGuaAACGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 13860 | 0.71 | 0.409337 |
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Target: 5'- cGCAUGG-GCCGCacGGgacgccgCAUggGCCGCc -3' miRNA: 3'- uCGUAUCuCGGCG--CCa------GUAaaCGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 13803 | 0.71 | 0.390405 |
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Target: 5'- cGCA-GGGGCCGCcG-CAUggGCCGCa -3' miRNA: 3'- uCGUaUCUCGGCGcCaGUAaaCGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 31960 | 0.72 | 0.354301 |
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Target: 5'- -uCGUGGGGCgCGCGGaCAUgaucGCCGCa -3' miRNA: 3'- ucGUAUCUCG-GCGCCaGUAaa--CGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 26718 | 0.73 | 0.304698 |
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Target: 5'- cGCAUGGGacGCCGCGGaCAag-GCCGUu -3' miRNA: 3'- uCGUAUCU--CGGCGCCaGUaaaCGGCG- -5' |
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| 33045 | 3' | -53.9 | NC_007497.1 | + | 14877 | 1.11 | 0.000665 |
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Target: 5'- gAGCAUAGAGCCGCGGUCAUUUGCCGCc -3' miRNA: 3'- -UCGUAUCUCGGCGCCAGUAAACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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