Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33046 | 5' | -60.1 | NC_007497.1 | + | 31306 | 0.66 | 0.419315 |
Target: 5'- -gGCGGCgCUUCCGaCGAUGgcucgAC-GGCa -3' miRNA: 3'- agCGCCG-GGAGGC-GCUACa----UGaCCGc -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 13806 | 0.66 | 0.401068 |
Target: 5'- gUGCGGCCC-CUGCGgcGUccccuGC-GGCGu -3' miRNA: 3'- aGCGCCGGGaGGCGCuaCA-----UGaCCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 13740 | 0.66 | 0.401068 |
Target: 5'- gUGCGGCCC-CUGCGgcGUccccuGC-GGCGu -3' miRNA: 3'- aGCGCCGGGaGGCGCuaCA-----UGaCCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 30767 | 0.66 | 0.392136 |
Target: 5'- gUCGCGGCgUagUCGCGgcGUuaAUUGGCGa -3' miRNA: 3'- -AGCGCCGgGa-GGCGCuaCA--UGACCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 27721 | 0.67 | 0.374665 |
Target: 5'- aUCGCGcGCUa-CUGCGAUGUGaUGGUGu -3' miRNA: 3'- -AGCGC-CGGgaGGCGCUACAUgACCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 11624 | 0.67 | 0.366129 |
Target: 5'- aUUGCGGCgC-CCaGCGAUG-AgUGGCGu -3' miRNA: 3'- -AGCGCCGgGaGG-CGCUACaUgACCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 9640 | 0.67 | 0.341336 |
Target: 5'- -gGCGcGCCggCCGCGAcGUcgGCUGGCu -3' miRNA: 3'- agCGC-CGGgaGGCGCUaCA--UGACCGc -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 32704 | 0.67 | 0.341336 |
Target: 5'- aUCGCGGaUCUCgCGCGGUucGCUGcGCGa -3' miRNA: 3'- -AGCGCCgGGAG-GCGCUAcaUGAC-CGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 9613 | 0.67 | 0.34053 |
Target: 5'- aUUGuCGGCCgUCCGCG-UGUACUcccgauuuccuucGGCc -3' miRNA: 3'- -AGC-GCCGGgAGGCGCuACAUGA-------------CCGc -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 7143 | 0.68 | 0.333346 |
Target: 5'- -aGCuGCCCUCC-CGGUaugaagACUGGCGg -3' miRNA: 3'- agCGcCGGGAGGcGCUAca----UGACCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 4065 | 0.68 | 0.310204 |
Target: 5'- cUCGCGuaCCgCCGCGAUGaGCUcGCGc -3' miRNA: 3'- -AGCGCcgGGaGGCGCUACaUGAcCGC- -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 39629 | 0.68 | 0.30203 |
Target: 5'- uUCGCGGCauuggcaCCgcCCGCGAUGUACguaaGCa -3' miRNA: 3'- -AGCGCCG-------GGa-GGCGCUACAUGac--CGc -5' |
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33046 | 5' | -60.1 | NC_007497.1 | + | 15130 | 1.09 | 0.000252 |
Target: 5'- aUCGCGGCCCUCCGCGAUGUACUGGCGg -3' miRNA: 3'- -AGCGCCGGGAGGCGCUACAUGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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