Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33048 | 3' | -55.5 | NC_007497.1 | + | 44044 | 0.66 | 0.670099 |
Target: 5'- cGCGGCCGGaaucaugcugACGGCGGucauuuuguGUUGGcGCgAGGa -3' miRNA: 3'- -UGCUGGCU----------UGCCGCC---------UAACU-CGgUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 42496 | 0.66 | 0.65902 |
Target: 5'- gACGGCUcGACGuGCGGAcgagcUUGGGCCu-- -3' miRNA: 3'- -UGCUGGcUUGC-CGCCU-----AACUCGGucu -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 4958 | 0.67 | 0.590165 |
Target: 5'- uGCGGCCGu-CGGCGGAggcaacagcagGAGUCguuGGAg -3' miRNA: 3'- -UGCUGGCuuGCCGCCUaa---------CUCGG---UCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 32513 | 0.67 | 0.576928 |
Target: 5'- uGCGACCG-GCGGCcacgucacgGGGCCGGGc -3' miRNA: 3'- -UGCUGGCuUGCCGccuaa----CUCGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 28614 | 0.68 | 0.537676 |
Target: 5'- gGCGGCgGcGGCGGUGGAgaGucGCCGGAc -3' miRNA: 3'- -UGCUGgC-UUGCCGCCUaaCu-CGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 29908 | 0.68 | 0.526929 |
Target: 5'- uCGugCGGacaGCGGCGGGUgagUGuauuuGCCGGGg -3' miRNA: 3'- uGCugGCU---UGCCGCCUA---ACu----CGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 14961 | 0.68 | 0.516263 |
Target: 5'- cCGGCCGGAaaaGGCGccg-GAGCCGGu -3' miRNA: 3'- uGCUGGCUUg--CCGCcuaaCUCGGUCu -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 26435 | 0.69 | 0.484818 |
Target: 5'- aGCGAaaccaCGGACGGCGGug-GAGUCGu- -3' miRNA: 3'- -UGCUg----GCUUGCCGCCuaaCUCGGUcu -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 36850 | 0.69 | 0.47454 |
Target: 5'- uCGAuCCGcugcgcgaGGCGGCGGGcgucGAGCCGGAc -3' miRNA: 3'- uGCU-GGC--------UUGCCGCCUaa--CUCGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 36771 | 0.7 | 0.400323 |
Target: 5'- cCGGCuCGAcgcccgccgccucgcGCaGCGGAUcGAGCCAGAa -3' miRNA: 3'- uGCUG-GCU---------------UGcCGCCUAaCUCGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 35641 | 0.7 | 0.386569 |
Target: 5'- gUGGCCGAcgcgaucGCGcGCGGcaUGGGCCGGGg -3' miRNA: 3'- uGCUGGCU-------UGC-CGCCuaACUCGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 42603 | 0.71 | 0.369601 |
Target: 5'- -aGACCGAcuggcccugcGCgGGCGGAUUGAgcGCCAuGAg -3' miRNA: 3'- ugCUGGCU----------UG-CCGCCUAACU--CGGU-CU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 31821 | 0.71 | 0.360878 |
Target: 5'- uGCGGCCgGAGCGGCGGcgU---CCAGAa -3' miRNA: 3'- -UGCUGG-CUUGCCGCCuaAcucGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 33467 | 0.72 | 0.296348 |
Target: 5'- cGCGGgCGGGCGGCGcccGUUccGAGCCGGAa -3' miRNA: 3'- -UGCUgGCUUGCCGCc--UAA--CUCGGUCU- -5' |
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33048 | 3' | -55.5 | NC_007497.1 | + | 16159 | 1.08 | 0.000882 |
Target: 5'- cACGACCGAACGGCGGAUUGAGCCAGAc -3' miRNA: 3'- -UGCUGGCUUGCCGCCUAACUCGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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