Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33048 | 5' | -52.7 | NC_007497.1 | + | 36592 | 0.66 | 0.798935 |
Target: 5'- -aUCGUGUGcCGGCCCGUacgccUCGGCGa -3' miRNA: 3'- uaAGCGCGUaGUUGGGCAac---AGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 42371 | 0.66 | 0.798935 |
Target: 5'- -cUCGCGUcucgCGACCCGUgccaccauUCAGCu -3' miRNA: 3'- uaAGCGCGua--GUUGGGCAac------AGUCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 17451 | 0.66 | 0.78879 |
Target: 5'- -aUCGUGaAUC-ACCCGaaaugGUCGGCGg -3' miRNA: 3'- uaAGCGCgUAGuUGGGCaa---CAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 42009 | 0.66 | 0.784682 |
Target: 5'- -cUCGCGCGccggCAACUCGaucuuguccccgGUCGGCGu -3' miRNA: 3'- uaAGCGCGUa---GUUGGGCaa----------CAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 28593 | 0.66 | 0.778468 |
Target: 5'- -aUC-CGCuUCAACCCGgauUCGGCGu -3' miRNA: 3'- uaAGcGCGuAGUUGGGCaacAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 19257 | 0.66 | 0.778468 |
Target: 5'- -cUCGCGU-UCAGCCU-UUGUCAGgCGu -3' miRNA: 3'- uaAGCGCGuAGUUGGGcAACAGUC-GC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 42150 | 0.66 | 0.757344 |
Target: 5'- --cCGCGcCAUCGACUCGccGUCgAGCa -3' miRNA: 3'- uaaGCGC-GUAGUUGGGCaaCAG-UCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 29928 | 0.66 | 0.746567 |
Target: 5'- cGUUCGUcCAUaaaaAACCCGccaGUCGGCGg -3' miRNA: 3'- -UAAGCGcGUAg---UUGGGCaa-CAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 28856 | 0.67 | 0.74004 |
Target: 5'- -gUCGCGCAaauguugagcccgcaUCAGCCCGgccUGgcuGGCGu -3' miRNA: 3'- uaAGCGCGU---------------AGUUGGGCa--ACag-UCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 3947 | 0.67 | 0.735664 |
Target: 5'- cUUCGUgccgGCGUCGACCCGca-UCGGCc -3' miRNA: 3'- uAAGCG----CGUAGUUGGGCaacAGUCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 41243 | 0.67 | 0.724649 |
Target: 5'- --cCGCGCca-AGCCgGUgGUCAGCGc -3' miRNA: 3'- uaaGCGCGuagUUGGgCAaCAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 31538 | 0.67 | 0.724649 |
Target: 5'- -aUCGCGUccCAACuugguCCGgcGUCAGCGu -3' miRNA: 3'- uaAGCGCGuaGUUG-----GGCaaCAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 1143 | 0.67 | 0.713533 |
Target: 5'- cUUCGCGCGgaUCAugCCGgccUGccCAGCa -3' miRNA: 3'- uAAGCGCGU--AGUugGGCa--ACa-GUCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 3605 | 0.67 | 0.691054 |
Target: 5'- -aUCGgGCGUguagAACgUGUUGUCGGCGa -3' miRNA: 3'- uaAGCgCGUAg---UUGgGCAACAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 44428 | 0.68 | 0.668328 |
Target: 5'- uUUCGcCGCAUCGAUCCGcaauuUCGGCu -3' miRNA: 3'- uAAGC-GCGUAGUUGGGCaac--AGUCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 2254 | 0.68 | 0.668328 |
Target: 5'- uAUUUGCGCGU--GCCCGgUGUCuGCc -3' miRNA: 3'- -UAAGCGCGUAguUGGGCaACAGuCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 26955 | 0.68 | 0.633992 |
Target: 5'- --cCGCGCAgcuguACCgCGUcGUCAGCGu -3' miRNA: 3'- uaaGCGCGUagu--UGG-GCAaCAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 37662 | 0.69 | 0.622528 |
Target: 5'- -aUCGCGUggaAUCGACCggcaUGUucucUGUCGGCGg -3' miRNA: 3'- uaAGCGCG---UAGUUGG----GCA----ACAGUCGC- -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 39197 | 0.73 | 0.397297 |
Target: 5'- --gCGCGCAUCGGCuCCGgauUCAGCa -3' miRNA: 3'- uaaGCGCGUAGUUG-GGCaacAGUCGc -5' |
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33048 | 5' | -52.7 | NC_007497.1 | + | 29016 | 0.75 | 0.271854 |
Target: 5'- ---aGCGaaCGUCAGCCCGUUGUCGcGCGc -3' miRNA: 3'- uaagCGC--GUAGUUGGGCAACAGU-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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