Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33050 | 3' | -53.1 | NC_007497.1 | + | 25594 | 0.66 | 0.80125 |
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Target: 5'- uCAUCGAGCCGCCguugUUCGggucgaCCaCGgcGAa -3' miRNA: 3'- -GUAGCUUGGCGG----AAGUag----GG-GCauCU- -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 4041 | 0.66 | 0.760427 |
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Target: 5'- -cUCG-GCCGCCg-CGUCCCCGa--- -3' miRNA: 3'- guAGCuUGGCGGaaGUAGGGGCaucu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 42552 | 0.67 | 0.749845 |
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Target: 5'- aCGUCGAGCCGUCauUUCAggCCUCGa--- -3' miRNA: 3'- -GUAGCUUGGCGG--AAGUa-GGGGCaucu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 34200 | 0.69 | 0.605239 |
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Target: 5'- gGUCGAguGCCGCCUg-AUCCUCGUc-- -3' miRNA: 3'- gUAGCU--UGGCGGAagUAGGGGCAucu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 5540 | 0.71 | 0.505879 |
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Target: 5'- cCAUCaGGACCGCCUUCGccggCCCCu---- -3' miRNA: 3'- -GUAG-CUUGGCGGAAGUa---GGGGcaucu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 28946 | 0.71 | 0.463917 |
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Target: 5'- -uUCGAAUCGCC-UCGUCCCCu---- -3' miRNA: 3'- guAGCUUGGCGGaAGUAGGGGcaucu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 17017 | 0.73 | 0.376794 |
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Target: 5'- -uUCGAacGCCGCCcgUUCGUCCgCCGgcGAg -3' miRNA: 3'- guAGCU--UGGCGG--AAGUAGG-GGCauCU- -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 24605 | 0.75 | 0.27898 |
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Target: 5'- uUAUCGGACgGCCUUCG-CCgCCGUGGc -3' miRNA: 3'- -GUAGCUUGgCGGAAGUaGG-GGCAUCu -5' |
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| 33050 | 3' | -53.1 | NC_007497.1 | + | 16946 | 1.09 | 0.001338 |
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Target: 5'- aCAUCGAACCGCCUUCAUCCCCGUAGAc -3' miRNA: 3'- -GUAGCUUGGCGGAAGUAGGGGCAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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