Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33053 | 3' | -49.2 | NC_007497.1 | + | 39549 | 0.66 | 0.937014 |
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Target: 5'- -aGAcCAGAUCGAUgAUgcCGUCCGAc- -3' miRNA: 3'- ucCUuGUCUAGCUAgUA--GCAGGCUug -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 24320 | 0.66 | 0.931364 |
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Target: 5'- gGGGAACAGAUgCGAgcgaGUCcaaccgaaGUCgCGAGCu -3' miRNA: 3'- -UCCUUGUCUA-GCUag--UAG--------CAG-GCUUG- -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 33177 | 0.67 | 0.899252 |
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Target: 5'- aAGGAacgcagcgcGCAGAUCGAcgcCGagcuggccgacuacgCGUCCGAGCg -3' miRNA: 3'- -UCCU---------UGUCUAGCUa--GUa--------------GCAGGCUUG- -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 23209 | 0.71 | 0.726485 |
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Target: 5'- cGGcaAGCAGAUCGAgggCGUCGccggcugCCGGGCu -3' miRNA: 3'- uCC--UUGUCUAGCUa--GUAGCa------GGCUUG- -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 37361 | 0.71 | 0.715245 |
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Target: 5'- --uGGCAGggCGGUCAgUCGUCCGAGa -3' miRNA: 3'- uccUUGUCuaGCUAGU-AGCAGGCUUg -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 8076 | 0.72 | 0.692505 |
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Target: 5'- uGGGAGCuAGGUCGAUCAaagcacUCGaaUCCGGugACa -3' miRNA: 3'- -UCCUUG-UCUAGCUAGU------AGC--AGGCU--UG- -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 19893 | 0.72 | 0.642868 |
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Target: 5'- cAGGGACGGGUCaucgagucaggccaGAcCGUCGUgCGAGCa -3' miRNA: 3'- -UCCUUGUCUAG--------------CUaGUAGCAgGCUUG- -5' |
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| 33053 | 3' | -49.2 | NC_007497.1 | + | 20868 | 1.1 | 0.003121 |
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Target: 5'- gAGGAACAGAUCGAUCAUCGUCCGAACc -3' miRNA: 3'- -UCCUUGUCUAGCUAGUAGCAGGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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