Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33054 | 3' | -51.5 | NC_007497.1 | + | 13617 | 0.68 | 0.707467 |
Target: 5'- cACUGAggaAGAGCGGgccGCcGCAUGGGc -3' miRNA: 3'- uUGACUag-UCUCGUCau-CG-CGUACUC- -5' |
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33054 | 3' | -51.5 | NC_007497.1 | + | 21338 | 1.06 | 0.002203 |
Target: 5'- gAACUGAUCAGAGCAGUAGCGCAUGAGc -3' miRNA: 3'- -UUGACUAGUCUCGUCAUCGCGUACUC- -5' |
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33054 | 3' | -51.5 | NC_007497.1 | + | 35781 | 0.66 | 0.825625 |
Target: 5'- aGAC-GAUCAGcaaGGCGGUGGCGCu---- -3' miRNA: 3'- -UUGaCUAGUC---UCGUCAUCGCGuacuc -5' |
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33054 | 3' | -51.5 | NC_007497.1 | + | 37910 | 0.66 | 0.825625 |
Target: 5'- cAGgUGGUCGGAGguGggcuuugAGCGCAUuGAc -3' miRNA: 3'- -UUgACUAGUCUCguCa------UCGCGUA-CUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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