Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33054 | 5' | -51 | NC_007497.1 | + | 21678 | 0.66 | 0.861854 |
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Target: 5'- -cCCuAUUgACGACgccgCCGCUGAUCGc -3' miRNA: 3'- aaGGcUAAgUGUUGaa--GGCGGCUAGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 27484 | 0.67 | 0.844194 |
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Target: 5'- -aCCGGaUCugACGGCaugUCCGCCGAUgCGa -3' miRNA: 3'- aaGGCUaAG--UGUUGa--AGGCGGCUA-GC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 32564 | 0.67 | 0.844194 |
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Target: 5'- gUCgGAUggcgUCGCGGCcgCCGCCGG-CGg -3' miRNA: 3'- aAGgCUA----AGUGUUGaaGGCGGCUaGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 29771 | 0.67 | 0.824601 |
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Target: 5'- aUCCGAacggCACGGCgcagCCgagcaugGCCGAUCGu -3' miRNA: 3'- aAGGCUaa--GUGUUGaa--GG-------CGGCUAGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 33698 | 0.68 | 0.785698 |
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Target: 5'- gUUCCGAcgugCGcCGGCUUCCGCCu-UCGc -3' miRNA: 3'- -AAGGCUaa--GU-GUUGAAGGCGGcuAGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 36543 | 0.68 | 0.785698 |
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Target: 5'- -cCCGGUcUGCGGCgaggCCGCCGAgcUCGa -3' miRNA: 3'- aaGGCUAaGUGUUGaa--GGCGGCU--AGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 34304 | 0.68 | 0.775264 |
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Target: 5'- gUUCCGAcgUUCGgaGACggUgCGCCGAUCGu -3' miRNA: 3'- -AAGGCU--AAGUg-UUGa-AgGCGGCUAGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 39592 | 0.71 | 0.618065 |
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Target: 5'- -aCCGcuGUUCACGACUUCgauggCGCCGAaCGg -3' miRNA: 3'- aaGGC--UAAGUGUUGAAG-----GCGGCUaGC- -5' |
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| 33054 | 5' | -51 | NC_007497.1 | + | 21297 | 1.08 | 0.002489 |
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Target: 5'- uUUCCGAUUCACAACUUCCGCCGAUCGg -3' miRNA: 3'- -AAGGCUAAGUGUUGAAGGCGGCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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