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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33055 | 3' | -54.6 | NC_007497.1 | + | 1743 | 0.66 | 0.678644 |
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Target: 5'- -gGCGCgaUGuGUGUCCGGUGC-CGUUc -3' miRNA: 3'- gaCGCGaaGC-UACAGGCUACGaGCGA- -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 28880 | 0.66 | 0.656204 |
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Target: 5'- -gGCGCUUCGAUGaugcUCggCGGUGUcgCGCa -3' miRNA: 3'- gaCGCGAAGCUAC----AG--GCUACGa-GCGa -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 38358 | 0.68 | 0.577485 |
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Target: 5'- aUGCGCaucucgcugUCGAUGUCCucccgGAaccugauacggaUGCUCGCg -3' miRNA: 3'- gACGCGa--------AGCUACAGG-----CU------------ACGAGCGa -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 2410 | 0.68 | 0.530058 |
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Target: 5'- cCUGUGCcgCGAUGUcgcgcuuagcagugCCGGUGCgcCGCUg -3' miRNA: 3'- -GACGCGaaGCUACA--------------GGCUACGa-GCGA- -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 28640 | 0.7 | 0.419577 |
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Target: 5'- uCUGCGUa--GAUGcCCGA-GCUCGCg -3' miRNA: 3'- -GACGCGaagCUACaGGCUaCGAGCGa -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 41709 | 0.71 | 0.409967 |
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Target: 5'- gCUGCGCcgaugcugCGGUGUUCGAgaacUGCuUCGCUg -3' miRNA: 3'- -GACGCGaa------GCUACAGGCU----ACG-AGCGA- -5' |
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| 33055 | 3' | -54.6 | NC_007497.1 | + | 21435 | 1.08 | 0.000984 |
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Target: 5'- uCUGCGCUUCGAUGUCCGAUGCUCGCUg -3' miRNA: 3'- -GACGCGAAGCUACAGGCUACGAGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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