Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33056 | 3' | -56.6 | NC_007497.1 | + | 37855 | 0.66 | 0.631561 |
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Target: 5'- gUCGGGggcgccaaaggcuACGUGUCCGUcGCGAGUUc- -3' miRNA: 3'- aGGCCU-------------UGCACAGGCGcUGCUCGAcu -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 6205 | 0.66 | 0.621697 |
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Target: 5'- aCCGGAuuuuggagGCGUGggCGCG-CGAGCg-- -3' miRNA: 3'- aGGCCU--------UGCACagGCGCuGCUCGacu -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 31163 | 0.66 | 0.599815 |
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Target: 5'- -aCGGcACGuUGUCCGCGAacguCGGGCg-- -3' miRNA: 3'- agGCCuUGC-ACAGGCGCU----GCUCGacu -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 38210 | 0.67 | 0.556445 |
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Target: 5'- gCCGGcgcgAACGUGaUCCGgGACGAgGCg-- -3' miRNA: 3'- aGGCC----UUGCAC-AGGCgCUGCU-CGacu -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 34664 | 0.67 | 0.535087 |
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Target: 5'- -aCGGAGCGUG-CCGCGGuCGcGCa-- -3' miRNA: 3'- agGCCUUGCACaGGCGCU-GCuCGacu -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 23684 | 0.68 | 0.518212 |
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Target: 5'- gCCGGcggccGCGUG-CUGCccggacuggugaaacGGCGAGCUGAg -3' miRNA: 3'- aGGCCu----UGCACaGGCG---------------CUGCUCGACU- -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 36246 | 0.7 | 0.361084 |
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Target: 5'- -aUGGcAACGaGUCUGCG-CGAGCUGAu -3' miRNA: 3'- agGCC-UUGCaCAGGCGCuGCUCGACU- -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 9637 | 0.71 | 0.352626 |
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Target: 5'- cCCGGcGCGccggCCGCGACGucGGCUGGc -3' miRNA: 3'- aGGCCuUGCaca-GGCGCUGC--UCGACU- -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 23259 | 0.75 | 0.186538 |
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Target: 5'- -gCGGGAUGgcgGgcggCUGCGACGAGCUGGu -3' miRNA: 3'- agGCCUUGCa--Ca---GGCGCUGCUCGACU- -5' |
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| 33056 | 3' | -56.6 | NC_007497.1 | + | 21694 | 1.09 | 0.000676 |
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Target: 5'- uUCCGGAACGUGUCCGCGACGAGCUGAc -3' miRNA: 3'- -AGGCCUUGCACAGGCGCUGCUCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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