Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33056 | 5' | -57.5 | NC_007497.1 | + | 23104 | 0.72 | 0.222016 |
Target: 5'- uCGUuaGCGCGUCGUAGUCGcGCucgcacugcugGCCGg -3' miRNA: 3'- -GUAcgCGCGCAGCAUCAGCuCG-----------CGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 3532 | 0.66 | 0.553818 |
Target: 5'- --cGCGCGCGUCGUcuugccaauGccccuucaccUCGuGCGCCu -3' miRNA: 3'- guaCGCGCGCAGCAu--------C----------AGCuCGCGGu -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 16227 | 0.66 | 0.521787 |
Target: 5'- -cUGUGUGCuUCGUucgCGGGCGCCc -3' miRNA: 3'- guACGCGCGcAGCAucaGCUCGCGGu -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 41069 | 0.66 | 0.511267 |
Target: 5'- --cGCGCGCGcCGauGUUGcgaAGCGCCGc -3' miRNA: 3'- guaCGCGCGCaGCauCAGC---UCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 42616 | 0.68 | 0.430754 |
Target: 5'- -cUGCGCGgG-CGga-UUGAGCGCCAu -3' miRNA: 3'- guACGCGCgCaGCaucAGCUCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 27908 | 0.68 | 0.402522 |
Target: 5'- --aGCGCGCGUCGaacGUcauacccaCGAGCGCa- -3' miRNA: 3'- guaCGCGCGCAGCau-CA--------GCUCGCGgu -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 32237 | 0.66 | 0.531326 |
Target: 5'- --gGcCGaCGCGUCGcagaugaUGG-CGAGCGCCGa -3' miRNA: 3'- guaC-GC-GCGCAGC-------AUCaGCUCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 30770 | 0.67 | 0.480264 |
Target: 5'- --cGUGuCGCGgCGUAGUCGcGGCGUUAa -3' miRNA: 3'- guaCGC-GCGCaGCAUCAGC-UCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 23571 | 0.67 | 0.480264 |
Target: 5'- -cUGgGCGCGgCGg---CGGGCGCCGg -3' miRNA: 3'- guACgCGCGCaGCaucaGCUCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 42788 | 0.67 | 0.470133 |
Target: 5'- aAUGCGCGaGUCGUuccGGUCGGGaaCGCa- -3' miRNA: 3'- gUACGCGCgCAGCA---UCAGCUC--GCGgu -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 27079 | 0.68 | 0.430754 |
Target: 5'- cCGU-CGgGCGUCGcacAGUCGcGCGCCGu -3' miRNA: 3'- -GUAcGCgCGCAGCa--UCAGCuCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 17339 | 0.69 | 0.349717 |
Target: 5'- aCGUGCGCGCGagGcuuUCGAGCGUg- -3' miRNA: 3'- -GUACGCGCGCagCaucAGCUCGCGgu -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 6938 | 0.73 | 0.199656 |
Target: 5'- --aGUGCGCGUCucGGUuucCGGGCGCCAu -3' miRNA: 3'- guaCGCGCGCAGcaUCA---GCUCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 21660 | 1.08 | 0.000558 |
Target: 5'- uCAUGCGCGCGUCGUAGUCGAGCGCCAa -3' miRNA: 3'- -GUACGCGCGCAGCAUCAGCUCGCGGU- -5' |
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33056 | 5' | -57.5 | NC_007497.1 | + | 39249 | 0.66 | 0.553818 |
Target: 5'- gAUGCGCGCGaUGaaGGUCGc-CGCCAg -3' miRNA: 3'- gUACGCGCGCaGCa-UCAGCucGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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