Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33057 | 3' | -53.1 | NC_007497.1 | + | 639 | 0.66 | 0.774653 |
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Target: 5'- -cGUAccccGCGCUGGGUGcGCacuGUCGUCg -3' miRNA: 3'- uuCAU----UGCGAUCCACcUGcu-CAGCGG- -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 42457 | 0.66 | 0.7385 |
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Target: 5'- ---cGGCGCggcgagcugaauGGUGGcACGGGUCGCg -3' miRNA: 3'- uucaUUGCGau----------CCACC-UGCUCAGCGg -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 6354 | 0.68 | 0.642263 |
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Target: 5'- -cGUucACGaCUacccAGGUGGuaGCGAGUCGCUg -3' miRNA: 3'- uuCAu-UGC-GA----UCCACC--UGCUCAGCGG- -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 15776 | 0.69 | 0.585475 |
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Target: 5'- gGAGUGACG---GGUGGGCGccGUCGCa -3' miRNA: 3'- -UUCAUUGCgauCCACCUGCu-CAGCGg -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 22090 | 0.69 | 0.585475 |
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Target: 5'- cGGUGACGgaAGucuaGUGGACGAaUCGCUu -3' miRNA: 3'- uUCAUUGCgaUC----CACCUGCUcAGCGG- -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 28615 | 0.69 | 0.585475 |
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Target: 5'- cGGcGGCgGCggcGGUGGA-GAGUCGCCg -3' miRNA: 3'- uUCaUUG-CGau-CCACCUgCUCAGCGG- -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 38541 | 0.71 | 0.486541 |
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Target: 5'- uGGGUGACGCacgacgAGGUuuGGGCGAG-CGUCc -3' miRNA: 3'- -UUCAUUGCGa-----UCCA--CCUGCUCaGCGG- -5' |
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| 33057 | 3' | -53.1 | NC_007497.1 | + | 22200 | 1.09 | 0.001209 |
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Target: 5'- gAAGUAACGCUAGGUGGACGAGUCGCCc -3' miRNA: 3'- -UUCAUUGCGAUCCACCUGCUCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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