Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33057 | 5' | -49.7 | NC_007497.1 | + | 23221 | 0.66 | 0.933085 |
Target: 5'- cGAGgGcgUCGcCgGCUgccGGGCUUCCGg -3' miRNA: 3'- cUUCgCuaAGCaGgUGA---UCUGAAGGC- -5' |
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33057 | 5' | -49.7 | NC_007497.1 | + | 22128 | 0.69 | 0.784254 |
Target: 5'- aGGGCGAcUCGUCCAcCUAGcguuACUUCUu -3' miRNA: 3'- cUUCGCUaAGCAGGU-GAUC----UGAAGGc -5' |
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33057 | 5' | -49.7 | NC_007497.1 | + | 44686 | 0.7 | 0.763068 |
Target: 5'- --cGCGAUggccgCGUCCuuAUUGGACUUgCCGg -3' miRNA: 3'- cuuCGCUAa----GCAGG--UGAUCUGAA-GGC- -5' |
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33057 | 5' | -49.7 | NC_007497.1 | + | 22166 | 1.09 | 0.002896 |
Target: 5'- aGAAGCGAUUCGUCCACUAGACUUCCGu -3' miRNA: 3'- -CUUCGCUAAGCAGGUGAUCUGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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