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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33061 | 5' | -56.7 | NC_007497.1 | + | 15238 | 0.66 | 0.578711 |
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Target: 5'- cGCGccuGUggcGCAUCCCGcGGAcGCCGGCc- -3' miRNA: 3'- cUGCu--CA---UGUAGGGC-CCU-UGGCCGac -5' |
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| 33061 | 5' | -56.7 | NC_007497.1 | + | 1856 | 0.66 | 0.5678 |
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Target: 5'- cGGCcGGUGCAUCUCGGaacuGAucGCCGGCc- -3' miRNA: 3'- -CUGcUCAUGUAGGGCC----CU--UGGCCGac -5' |
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| 33061 | 5' | -56.7 | NC_007497.1 | + | 37201 | 0.68 | 0.451795 |
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Target: 5'- gGACGAGUAguuucCGUCgaccgagaauucgCCGGGAuCCGGCa- -3' miRNA: 3'- -CUGCUCAU-----GUAG-------------GGCCCUuGGCCGac -5' |
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| 33061 | 5' | -56.7 | NC_007497.1 | + | 2267 | 0.68 | 0.442938 |
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Target: 5'- uGGCGAGccgugcuauuUGCGcgugCCCGGuGucuGCCGGCUGa -3' miRNA: 3'- -CUGCUC----------AUGUa---GGGCC-Cu--UGGCCGAC- -5' |
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| 33061 | 5' | -56.7 | NC_007497.1 | + | 24637 | 1.08 | 0.000622 |
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Target: 5'- uGACGAGUACAUCCCGGGAACCGGCUGg -3' miRNA: 3'- -CUGCUCAUGUAGGGCCCUUGGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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