Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33064 | 5' | -59 | NC_007497.1 | + | 15787 | 0.71 | 0.226983 |
Target: 5'- uGGGCGCc-GUCGcACGUUGaacaggucuGCAGCGCAg -3' miRNA: 3'- -UCCGCGcaCAGC-UGCGAC---------UGUCGCGU- -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 35686 | 0.71 | 0.226983 |
Target: 5'- -cGCGCuGUGUCGgccgauACGCcgGACAGCGCc -3' miRNA: 3'- ucCGCG-CACAGC------UGCGa-CUGUCGCGu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 17582 | 0.72 | 0.198978 |
Target: 5'- cGGCGCGUGcaaguUCcGCGCgGGCGGCGUc -3' miRNA: 3'- uCCGCGCAC-----AGcUGCGaCUGUCGCGu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 34066 | 0.72 | 0.188645 |
Target: 5'- gGGGCGCG---CGcACGCUGGCAGCGa- -3' miRNA: 3'- -UCCGCGCacaGC-UGCGACUGUCGCgu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 25925 | 1.08 | 0.000359 |
Target: 5'- aAGGCGCGUGUCGACGCUGACAGCGCAa -3' miRNA: 3'- -UCCGCGCACAGCUGCGACUGUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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