miRNA: 3'- -GUCGa--GCGa-CUAGCGGCUGUGCcacu -5'
miRNA: 3'- gUCGa---GCGACUA-GCGGcuGUGCCACU- -5'
miRNA: 3'- gUCGaGCGa--CUAGCGGCUGUGC---CACU- -5'
miRNA: 3'- gUCGAGCGAcUAGCGGCUGu---GCCaCU- -5'
miRNA: 3'- -GUCGAGCGACUAGCGGCUGUGCCACU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33068 | 3' | -56.7 | NC_007497.1 | + | 23534 | 0.71 | 0.300673 |
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Target: 5'- uCAGCgcgCGCgaGAUCGCCGACAUGa--- -3' miRNA: 3'- -GUCGa--GCGa-CUAGCGGCUGUGCcacu -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 41088 | 0.71 | 0.293225 |
|
Target: 5'- aAGCgccgCGCUGAUcCGCUu-CGCGGUGAc -3' miRNA: 3'- gUCGa---GCGACUA-GCGGcuGUGCCACU- -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 38225 | 0.71 | 0.285922 |
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Target: 5'- aGGCgCGCcacGGUCGCCGGCGCGaacGUGAu -3' miRNA: 3'- gUCGaGCGa--CUAGCGGCUGUGC---CACU- -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 14782 | 0.72 | 0.278764 |
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Target: 5'- uGGCUUGCUcGUCGCCGACugcuCGGcGGc -3' miRNA: 3'- gUCGAGCGAcUAGCGGCUGu---GCCaCU- -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 28061 | 1.09 | 0.000557 |
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Target: 5'- aCAGCUCGCUGAUCGCCGACACGGUGAa -3' miRNA: 3'- -GUCGAGCGACUAGCGGCUGUGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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