Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33069 | 3' | -54.5 | NC_007497.1 | + | 30553 | 0.66 | 0.731885 |
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Target: 5'- uCCAGG-CgGCCGG-UUUCgaacgGACGUCg -3' miRNA: 3'- uGGUCCuG-CGGCCaAAAGag---CUGCAG- -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 19926 | 0.66 | 0.699276 |
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Target: 5'- gACCgcgaGGGuuGCCGGUUUccaUCaGGCGUCg -3' miRNA: 3'- -UGG----UCCugCGGCCAAAag-AG-CUGCAG- -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 33862 | 0.66 | 0.683831 |
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Target: 5'- uACCGauGACGUCGGaugccucuuucagUUUCUCGACGUa -3' miRNA: 3'- -UGGUc-CUGCGGCCa------------AAAGAGCUGCAg -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 14512 | 0.67 | 0.632546 |
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Target: 5'- gGCCGGGaAUGCCGGg---C-CGcCGUCg -3' miRNA: 3'- -UGGUCC-UGCGGCCaaaaGaGCuGCAG- -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 9637 | 0.68 | 0.576811 |
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Target: 5'- cCCGGcGCGCCGGccg---CGACGUCg -3' miRNA: 3'- uGGUCcUGCGGCCaaaagaGCUGCAG- -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 33081 | 0.68 | 0.554783 |
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Target: 5'- cGCUcGGACGCguaguCGGccagCUCGGCGUCg -3' miRNA: 3'- -UGGuCCUGCG-----GCCaaaaGAGCUGCAG- -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 32348 | 0.69 | 0.505178 |
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Target: 5'- aACgCAGGACGCCGGgcUUCcgaaccgcagaagccUUGACGa- -3' miRNA: 3'- -UG-GUCCUGCGGCCaaAAG---------------AGCUGCag -5' |
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| 33069 | 3' | -54.5 | NC_007497.1 | + | 29463 | 1.1 | 0.000863 |
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Target: 5'- uACCAGGACGCCGGUUUUCUCGACGUCg -3' miRNA: 3'- -UGGUCCUGCGGCCAAAAGAGCUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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