Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33070 | 5' | -56 | NC_007497.1 | + | 628 | 0.66 | 0.642539 |
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Target: 5'- -cUGGGUgCGCaCUGUCGUCGCaGCa -3' miRNA: 3'- caGCUCAaGUGcGACAGCAGCGgCGa -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 4504 | 0.66 | 0.587148 |
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Target: 5'- cGUCGAaaacgACGCUGUUGUaggUGCCGCa -3' miRNA: 3'- -CAGCUcaag-UGCGACAGCA---GCGGCGa -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 186 | 0.67 | 0.532696 |
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Target: 5'- gGUCGGG-UCGaGCUGgagccgucUGUCGCCGCg -3' miRNA: 3'- -CAGCUCaAGUgCGACa-------GCAGCGGCGa -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 10462 | 0.68 | 0.469953 |
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Target: 5'- aGUUGAGcccacgaUCACGCUGcCGUCugGCUGCUc -3' miRNA: 3'- -CAGCUCa------AGUGCGACaGCAG--CGGCGA- -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 14770 | 0.69 | 0.420676 |
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Target: 5'- cGUCGAuccgUCugGCuUGcUCGUCGCCGaCUg -3' miRNA: 3'- -CAGCUca--AGugCG-AC-AGCAGCGGC-GA- -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 41530 | 0.73 | 0.245022 |
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Target: 5'- -aCGAGggCGCGCUGUUG-CGCgGCa -3' miRNA: 3'- caGCUCaaGUGCGACAGCaGCGgCGa -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 22929 | 0.8 | 0.080631 |
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Target: 5'- -aCGAG-UCAgggcCGCUGUCGUCGCCGCc -3' miRNA: 3'- caGCUCaAGU----GCGACAGCAGCGGCGa -5' |
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| 33070 | 5' | -56 | NC_007497.1 | + | 30288 | 1.07 | 0.000996 |
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Target: 5'- gGUCGAGUUCACGCUGUCGUCGCCGCUc -3' miRNA: 3'- -CAGCUCAAGUGCGACAGCAGCGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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