Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33071 | 5' | -53 | NC_007497.1 | + | 15577 | 0.66 | 0.771057 |
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Target: 5'- gCGAUCGCCcuuuuucugGCGAUGcagGUgGUGCUGg -3' miRNA: 3'- -GCUAGUGG---------CGUUACacaCAgCGCGAC- -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 41722 | 0.68 | 0.672692 |
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Target: 5'- aGGUCGCgCGCAuguacgGUGUGcCGCGUc- -3' miRNA: 3'- gCUAGUG-GCGUua----CACACaGCGCGac -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 34637 | 0.7 | 0.537584 |
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Target: 5'- gCGGUCGCCGCGGUGgcgccucGUucggCGCGCUc -3' miRNA: 3'- -GCUAGUGGCGUUACa------CAca--GCGCGAc -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 26804 | 0.7 | 0.526621 |
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Target: 5'- uCGAUCGCCGCGuacuUGcccgGcucaUGUCGCGUUGc -3' miRNA: 3'- -GCUAGUGGCGUu---ACa---C----ACAGCGCGAC- -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 1734 | 0.73 | 0.403374 |
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Target: 5'- gGAUCACucggCGCGAUGUGUGUCcgGUGCc- -3' miRNA: 3'- gCUAGUG----GCGUUACACACAG--CGCGac -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 37809 | 0.73 | 0.393939 |
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Target: 5'- -aAUCGCCGUAcuacGUG-GUGUCGCGCg- -3' miRNA: 3'- gcUAGUGGCGU----UACaCACAGCGCGac -5' |
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| 33071 | 5' | -53 | NC_007497.1 | + | 30476 | 1.1 | 0.001079 |
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Target: 5'- aCGAUCACCGCAAUGUGUGUCGCGCUGg -3' miRNA: 3'- -GCUAGUGGCGUUACACACAGCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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