Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33073 | 5' | -53.5 | NC_007497.1 | + | 21562 | 0.66 | 0.762153 |
Target: 5'- ------gCGUGGGCgcagcucGUCGACGGCGu -3' miRNA: 3'- ccuaaagGCGCCCGaa-----CAGCUGUCGU- -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 11095 | 0.66 | 0.730123 |
Target: 5'- -----aCCGCcuugcgGGGUUUGUCGAUGGCc -3' miRNA: 3'- ccuaaaGGCG------CCCGAACAGCUGUCGu -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 3255 | 0.67 | 0.697164 |
Target: 5'- cGGAgaccggCC-CGGGCc--UCGACAGCAu -3' miRNA: 3'- -CCUaaa---GGcGCCCGaacAGCUGUCGU- -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 40746 | 0.69 | 0.56242 |
Target: 5'- cGGA-UUCgCGaCGGGCaaGcUCGACAGCGc -3' miRNA: 3'- -CCUaAAG-GC-GCCCGaaC-AGCUGUCGU- -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 36183 | 0.76 | 0.239468 |
Target: 5'- aGGAUg--CGUGGGCUggcGUCGACGGCc -3' miRNA: 3'- -CCUAaagGCGCCCGAa--CAGCUGUCGu -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 32340 | 0.76 | 0.233149 |
Target: 5'- -----aCgGUGGGCUUGUUGGCGGCAg -3' miRNA: 3'- ccuaaaGgCGCCCGAACAGCUGUCGU- -5' |
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33073 | 5' | -53.5 | NC_007497.1 | + | 32733 | 1.11 | 0.000907 |
Target: 5'- uGGAUUUCCGCGGGCUUGUCGACAGCAu -3' miRNA: 3'- -CCUAAAGGCGCCCGAACAGCUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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