miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33081 5' -57.1 NC_007497.1 + 43493 0.67 0.540785
Target:  5'- -cGGGCGGUCGGUCaGUgCCGAa----- -3'
miRNA:   3'- guCCCGCCAGUCAG-CA-GGCUcuagcu -5'
33081 5' -57.1 NC_007497.1 + 34895 0.67 0.540785
Target:  5'- gCAGGGCGaGcUCgAG-CGUCCGAGGcgUCGc -3'
miRNA:   3'- -GUCCCGC-C-AG-UCaGCAGGCUCU--AGCu -5'
33081 5' -57.1 NC_007497.1 + 16431 0.67 0.496854
Target:  5'- --aGGUGGUCAGacuuggacgacgUGUCCGAGGUUGGc -3'
miRNA:   3'- gucCCGCCAGUCa-----------GCAGGCUCUAGCU- -5'
33081 5' -57.1 NC_007497.1 + 30309 0.69 0.429442
Target:  5'- uCGGGuccGCGGUCGGauuccCGUCCGGGAaauucaccgcgUCGAg -3'
miRNA:   3'- -GUCC---CGCCAGUCa----GCAGGCUCU-----------AGCU- -5'
33081 5' -57.1 NC_007497.1 + 25621 0.73 0.228197
Target:  5'- gCAGGGCGGUCAGgucuucauUCGUCaggcguuGAUCGGc -3'
miRNA:   3'- -GUCCCGCCAGUC--------AGCAGgcu----CUAGCU- -5'
33081 5' -57.1 NC_007497.1 + 37359 1.08 0.000698
Target:  5'- gCAGGGCGGUCAGUCGUCCGAGAUCGAc -3'
miRNA:   3'- -GUCCCGCCAGUCAGCAGGCUCUAGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.