Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33083 | 5' | -46.6 | NC_007497.1 | + | 20686 | 0.66 | 0.986405 |
Target: 5'- gGGGUGGGUUGcaggaacauGGCGCGGgAUCa-- -3' miRNA: 3'- gCCCAUUCAACu--------UUGCGUUgUAGcag -5' |
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33083 | 5' | -46.6 | NC_007497.1 | + | 42803 | 0.66 | 0.986405 |
Target: 5'- aCGuGGgcAGUUGGAaauGCGCGA-GUCGUUc -3' miRNA: 3'- -GC-CCauUCAACUU---UGCGUUgUAGCAG- -5' |
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33083 | 5' | -46.6 | NC_007497.1 | + | 19209 | 0.69 | 0.923124 |
Target: 5'- uGGGauAGUUG-AGCGUcaauGGCGUCGUCc -3' miRNA: 3'- gCCCauUCAACuUUGCG----UUGUAGCAG- -5' |
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33083 | 5' | -46.6 | NC_007497.1 | + | 38159 | 1.13 | 0.003674 |
Target: 5'- aCGGGUAAGUUGAAACGCAACAUCGUCa -3' miRNA: 3'- -GCCCAUUCAACUUUGCGUUGUAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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