Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33084 | 3' | -55.7 | NC_007497.1 | + | 2996 | 0.66 | 0.61407 |
Target: 5'- -cCGUggcagaaAUgGAGCGAgcAGGaGGAGGCCGa -3' miRNA: 3'- uaGCAg------UAgCUCGCU--UUC-CCUCCGGC- -5' |
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33084 | 3' | -55.7 | NC_007497.1 | + | 32600 | 0.67 | 0.536882 |
Target: 5'- cUCGUCGUCGcuucucGGCGGAcuggcGGGcGGCCu -3' miRNA: 3'- uAGCAGUAGC------UCGCUUu----CCCuCCGGc -5' |
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33084 | 3' | -55.7 | NC_007497.1 | + | 7695 | 0.69 | 0.423729 |
Target: 5'- -aCG-CAUCGGGCGGgucaucGAGGG-GGCUGa -3' miRNA: 3'- uaGCaGUAGCUCGCU------UUCCCuCCGGC- -5' |
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33084 | 3' | -55.7 | NC_007497.1 | + | 43047 | 0.7 | 0.404705 |
Target: 5'- cUCG-CAUCGAGCGcgcuGGGcuucuauacaAGGCCGg -3' miRNA: 3'- uAGCaGUAGCUCGCuuu-CCC----------UCCGGC- -5' |
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33084 | 3' | -55.7 | NC_007497.1 | + | 38602 | 1.07 | 0.000909 |
Target: 5'- cAUCGUCAUCGAGCGAAAGGGAGGCCGa -3' miRNA: 3'- -UAGCAGUAGCUCGCUUUCCCUCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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