Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 33087 | 5' | -54.9 | NC_007497.1 | + | 27902 | 0.66 | 0.692965 |
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Target: 5'- uGCguGUGCCGUGAAcuacgauccugagacGCGCaCGUacUCGGg -3' miRNA: 3'- -CGguUACGGCGCUU---------------CGUGgGCA--AGCC- -5' |
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| 33087 | 5' | -54.9 | NC_007497.1 | + | 3546 | 0.66 | 0.710372 |
|
Target: 5'- uGCCAAUGCCccuucaccucguGCGccucGAGCuggCCGUUCGc -3' miRNA: 3'- -CGGUUACGG------------CGC----UUCGug-GGCAAGCc -5' |
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| 33087 | 5' | -54.9 | NC_007497.1 | + | 26109 | 0.66 | 0.710372 |
|
Target: 5'- uGCCGAUcugGCCGCGAaaguAGUcCUCGUaugccgccucaUCGGu -3' miRNA: 3'- -CGGUUA---CGGCGCU----UCGuGGGCA-----------AGCC- -5' |
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| 33087 | 5' | -54.9 | NC_007497.1 | + | 32619 | 0.66 | 0.677608 |
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Target: 5'- cGCCAAUGCCGCuc-GCGCagcgaaCCGcgCGa -3' miRNA: 3'- -CGGUUACGGCGcuuCGUG------GGCaaGCc -5' |
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| 33087 | 5' | -54.9 | NC_007497.1 | + | 33318 | 0.66 | 0.721153 |
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Target: 5'- aCCGAUcugGCgGCGAAGCGCaCGaagcugUCGGa -3' miRNA: 3'- cGGUUA---CGgCGCUUCGUGgGCa-----AGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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