miRNA display CGI


Results 1 - 16 of 16 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33095 3' -50.6 NC_007497.1 + 11821 0.66 0.89511
Target:  5'- -gCGUaaCUAGCAGUUgAUCGuCGAuGCg -3'
miRNA:   3'- uaGCAa-GGUCGUCAAgUAGC-GCUuCG- -5'
33095 3' -50.6 NC_007497.1 + 36720 0.66 0.887482
Target:  5'- uGUCGagaccgcCCAGgGGUggCGUCGCGAauucGGCg -3'
miRNA:   3'- -UAGCaa-----GGUCgUCAa-GUAGCGCU----UCG- -5'
33095 3' -50.6 NC_007497.1 + 36883 0.66 0.877944
Target:  5'- uUCGUgccgccaccccgCCAGCgGGUUCAgaaUUGCGgcGCg -3'
miRNA:   3'- uAGCAa-----------GGUCG-UCAAGU---AGCGCuuCG- -5'
33095 3' -50.6 NC_007497.1 + 1967 0.66 0.854128
Target:  5'- -gCGggCCGGCacAGUUUcaaGCGAAGCa -3'
miRNA:   3'- uaGCaaGGUCG--UCAAGuagCGCUUCG- -5'
33095 3' -50.6 NC_007497.1 + 27494 0.67 0.835858
Target:  5'- cUCGUUCCGGUAugUCGgcaGCG-AGCg -3'
miRNA:   3'- uAGCAAGGUCGUcaAGUag-CGCuUCG- -5'
33095 3' -50.6 NC_007497.1 + 42661 0.67 0.835858
Target:  5'- --aGggCCAGuCGGUcuacgaGUCGCGggGCa -3'
miRNA:   3'- uagCaaGGUC-GUCAag----UAGCGCuuCG- -5'
33095 3' -50.6 NC_007497.1 + 27628 0.67 0.826359
Target:  5'- -gCGUUCaagguguuGCAGgaCAUCGCG-AGCg -3'
miRNA:   3'- uaGCAAGgu------CGUCaaGUAGCGCuUCG- -5'
33095 3' -50.6 NC_007497.1 + 41002 0.67 0.826359
Target:  5'- -gCGgcgUCGGCAGccucggUCAUCGCGAuggccGGCg -3'
miRNA:   3'- uaGCaa-GGUCGUCa-----AGUAGCGCU-----UCG- -5'
33095 3' -50.6 NC_007497.1 + 15166 0.67 0.816632
Target:  5'- -gCGcgCCAcGCGGUUCAaCGCaAAGCa -3'
miRNA:   3'- uaGCaaGGU-CGUCAAGUaGCGcUUCG- -5'
33095 3' -50.6 NC_007497.1 + 1713 0.68 0.764995
Target:  5'- -gCGcUCgGGCAGUUUcUCGCG-AGCg -3'
miRNA:   3'- uaGCaAGgUCGUCAAGuAGCGCuUCG- -5'
33095 3' -50.6 NC_007497.1 + 14954 0.69 0.720864
Target:  5'- gAUCGUUCCGGCAucagCGUCG-GcGGCg -3'
miRNA:   3'- -UAGCAAGGUCGUcaa-GUAGCgCuUCG- -5'
33095 3' -50.6 NC_007497.1 + 35013 0.7 0.663584
Target:  5'- aAUCGagCCGGCgaucaacgcGGacugCAUCGCGAGGCu -3'
miRNA:   3'- -UAGCaaGGUCG---------UCaa--GUAGCGCUUCG- -5'
33095 3' -50.6 NC_007497.1 + 25688 0.71 0.601989
Target:  5'- uGUCGgcCCAGCGGUUCGcCGUGGucgacccgaacaacGGCg -3'
miRNA:   3'- -UAGCaaGGUCGUCAAGUaGCGCU--------------UCG- -5'
33095 3' -50.6 NC_007497.1 + 22386 0.72 0.559363
Target:  5'- cAUCGcUUCCGGCcaaugCGUCGCGAAGa -3'
miRNA:   3'- -UAGC-AAGGUCGucaa-GUAGCGCUUCg -5'
33095 3' -50.6 NC_007497.1 + 39492 0.81 0.174115
Target:  5'- -gCGUguu-GCGGUUCAUCGCGAAGCg -3'
miRNA:   3'- uaGCAagguCGUCAAGUAGCGCUUCG- -5'
33095 3' -50.6 NC_007497.1 + 43428 1.12 0.00142
Target:  5'- gAUCGUUCCAGCAGUUCAUCGCGAAGCa -3'
miRNA:   3'- -UAGCAAGGUCGUCAAGUAGCGCUUCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.