Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33096 | 3' | -51.6 | NC_007497.1 | + | 13340 | 0.66 | 0.828301 |
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Target: 5'- ---gAGCGUGACGGuaaAGC-GCGGAGu- -3' miRNA: 3'- cuugUCGCACUGCU---UCGaCGCUUCuu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 13104 | 0.66 | 0.818583 |
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Target: 5'- cAGCgAGCGUGACGGuaaAGC-GCGggGc- -3' miRNA: 3'- cUUG-UCGCACUGCU---UCGaCGCuuCuu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 1294 | 0.66 | 0.818583 |
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Target: 5'- -uACGGCGaaacgaugaUGGCGAAGCgccUGCGggGu- -3' miRNA: 3'- cuUGUCGC---------ACUGCUUCG---ACGCuuCuu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 33412 | 0.67 | 0.745085 |
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Target: 5'- cGAGCAGCGgaucGCGcAGCUGCGcgucGAGGc -3' miRNA: 3'- -CUUGUCGCac--UGCuUCGACGC----UUCUu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 22977 | 0.67 | 0.733962 |
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Target: 5'- aAACAGCGgaauuGCGAGuGCUGCGAuGAu -3' miRNA: 3'- cUUGUCGCac---UGCUU-CGACGCUuCUu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 18770 | 0.68 | 0.722724 |
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Target: 5'- ---aAGCGcuggcGGCGAAGCUGCGcAGAu -3' miRNA: 3'- cuugUCGCa----CUGCUUCGACGCuUCUu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 40188 | 0.68 | 0.722724 |
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Target: 5'- cGAACGGCGUGAaGccGCUGCGc-GAu -3' miRNA: 3'- -CUUGUCGCACUgCuuCGACGCuuCUu -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 18634 | 0.73 | 0.390376 |
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Target: 5'- gGAGCGGCGUGGaaaGAGGCUGagaaaauccUGAAGGAg -3' miRNA: 3'- -CUUGUCGCACUg--CUUCGAC---------GCUUCUU- -5' |
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| 33096 | 3' | -51.6 | NC_007497.1 | + | 44424 | 1.06 | 0.00224 |
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Target: 5'- gGAACAGCGUGACGAAGCUGCGAAGAAg -3' miRNA: 3'- -CUUGUCGCACUGCUUCGACGCUUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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