Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33100 | 5' | -53.8 | NC_007497.1 | + | 43865 | 0.7 | 0.509625 |
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Target: 5'- gAUCaUCGAAGGCGUCUUCGGggUGAagCGCa -3' miRNA: 3'- -UGG-GGCUUCUGCGGAAGCU--ACUa-GCG- -5' |
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| 33100 | 5' | -53.8 | NC_007497.1 | + | 19196 | 0.72 | 0.427993 |
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Target: 5'- -aCCCGAAcaacGGCGgC-UCGAUGAUCGUg -3' miRNA: 3'- ugGGGCUU----CUGCgGaAGCUACUAGCG- -5' |
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| 33100 | 5' | -53.8 | NC_007497.1 | + | 17735 | 0.73 | 0.381066 |
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Target: 5'- gGCgCCCGcaguGGCGCCg--GGUGAUCGCu -3' miRNA: 3'- -UG-GGGCuu--CUGCGGaagCUACUAGCG- -5' |
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| 33100 | 5' | -53.8 | NC_007497.1 | + | 21675 | 0.73 | 0.337701 |
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Target: 5'- cGCCCCuauuGAcGACGCCgcCGcUGAUCGCu -3' miRNA: 3'- -UGGGG----CUuCUGCGGaaGCuACUAGCG- -5' |
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| 33100 | 5' | -53.8 | NC_007497.1 | + | 27928 | 0.74 | 0.305674 |
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Target: 5'- uCCCCGuAGACGCCUUUgGGUGcaCGCa -3' miRNA: 3'- uGGGGCuUCUGCGGAAG-CUACuaGCG- -5' |
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| 33100 | 5' | -53.8 | NC_007497.1 | + | 925 | 1.12 | 0.000712 |
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Target: 5'- cACCCCGAAGACGCCUUCGAUGAUCGCg -3' miRNA: 3'- -UGGGGCUUCUGCGGAAGCUACUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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