Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33102 | 5' | -55.9 | NC_007497.1 | + | 34856 | 0.71 | 0.369203 |
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Target: 5'- -gCCGCGgUcUCGCGCGcGAUCAcgGCCg -3' miRNA: 3'- agGGUGUgGaAGCGCGC-CUAGUa-CGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 28728 | 0.72 | 0.284513 |
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Target: 5'- gCCCGCGCCaUUCGCGCaucaacccguugucaGcGAUCGUGUa -3' miRNA: 3'- aGGGUGUGG-AAGCGCG---------------C-CUAGUACGg -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 30219 | 0.72 | 0.281637 |
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Target: 5'- gCCUugcauCACCUUCGCcgcgaaguagucGCGGAgcgaCAUGCCg -3' miRNA: 3'- aGGGu----GUGGAAGCG------------CGCCUa---GUACGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 27222 | 0.74 | 0.228893 |
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Target: 5'- aUCCguCACCUcggCGCGCGGcUCcgGCUg -3' miRNA: 3'- -AGGguGUGGAa--GCGCGCCuAGuaCGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 15235 | 0.75 | 0.195021 |
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Target: 5'- cCCCGCGCCUguggCGCaucccGCGGAcgCcgGCCg -3' miRNA: 3'- aGGGUGUGGAa---GCG-----CGCCUa-GuaCGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 27134 | 0.75 | 0.186759 |
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Target: 5'- gCCC-CGCCUU-GCGCGGAUCGgcaauagcgaucaccUGCCc -3' miRNA: 3'- aGGGuGUGGAAgCGCGCCUAGU---------------ACGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 36781 | 0.78 | 0.118492 |
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Target: 5'- gCCCGcCGCCUcgCGCaGCGGAUCGaGCCa -3' miRNA: 3'- aGGGU-GUGGAa-GCG-CGCCUAGUaCGG- -5' |
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| 33102 | 5' | -55.9 | NC_007497.1 | + | 1134 | 1.13 | 0.000376 |
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Target: 5'- cUCCCACACCUUCGCGCGGAUCAUGCCg -3' miRNA: 3'- -AGGGUGUGGAAGCGCGCCUAGUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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