Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33103 | 3' | -55 | NC_007497.1 | + | 2685 | 0.66 | 0.67927 |
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Target: 5'- aCCGUGCGgcGCGAGaucCCG-GUCUUcGCg -3' miRNA: 3'- gGGUACGU--UGCUCgc-GGCgCAGAA-CG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 40478 | 0.66 | 0.67927 |
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Target: 5'- -gUAUGCAGCGA-CGCUGCaacGUCUUcGCc -3' miRNA: 3'- ggGUACGUUGCUcGCGGCG---CAGAA-CG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 34826 | 0.67 | 0.646005 |
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Target: 5'- gCCUcgGCAcgcuCGAGCucgcccugcgcgGCCGCgGUCUcGCg -3' miRNA: 3'- -GGGuaCGUu---GCUCG------------CGGCG-CAGAaCG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 21827 | 0.67 | 0.634877 |
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Target: 5'- uCCCAaGCGACGGGCuGCCGa----UGCu -3' miRNA: 3'- -GGGUaCGUUGCUCG-CGGCgcagaACG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 14293 | 0.67 | 0.601516 |
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Target: 5'- gCgCAUGCAcugcgucgccaGCGAGCGgcCCGCGgCUcUGCa -3' miRNA: 3'- -GgGUACGU-----------UGCUCGC--GGCGCaGA-ACG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 29366 | 0.68 | 0.579392 |
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Target: 5'- gCCcgGUu-CGAGCGCCaCGUCgcgGCc -3' miRNA: 3'- gGGuaCGuuGCUCGCGGcGCAGaa-CG- -5' |
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| 33103 | 3' | -55 | NC_007497.1 | + | 4560 | 1.14 | 0.000374 |
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Target: 5'- gCCCAUGCAACGAGCGCCGCGUCUUGCg -3' miRNA: 3'- -GGGUACGUUGCUCGCGGCGCAGAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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