miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33103 5' -53.8 NC_007497.1 + 29722 0.66 0.700554
Target:  5'- uGGGCAUCgcggcccucCGCGAUGUaCUGgCGGGCc -3'
miRNA:   3'- -CUCGUAGaa-------GUGCUACA-GGC-GUCCGu -5'
33103 5' -53.8 NC_007497.1 + 6753 0.66 0.689308
Target:  5'- cGGGUAUCgcaacCGcCGGUGUCCGUguucggacgaaGGGCAa -3'
miRNA:   3'- -CUCGUAGaa---GU-GCUACAGGCG-----------UCCGU- -5'
33103 5' -53.8 NC_007497.1 + 27571 0.67 0.66665
Target:  5'- cGAGCGUCUgccugCGCGGgcggCUGCGGGgCGg -3'
miRNA:   3'- -CUCGUAGAa----GUGCUaca-GGCGUCC-GU- -5'
33103 5' -53.8 NC_007497.1 + 21093 0.68 0.608458
Target:  5'- cGGGCuAUCaaUCACGGUGUgcggguaCUGCGGGCGc -3'
miRNA:   3'- -CUCG-UAGa-AGUGCUACA-------GGCGUCCGU- -5'
33103 5' -53.8 NC_007497.1 + 30451 0.69 0.52754
Target:  5'- cGAGCA-CUUCgucgacuuuccugcGCGAUGcagagCCGCGGGCc -3'
miRNA:   3'- -CUCGUaGAAG--------------UGCUACa----GGCGUCCGu -5'
33103 5' -53.8 NC_007497.1 + 14920 0.7 0.445864
Target:  5'- cGAGUAUCcgCGCGAgugCUGCGGGCu -3'
miRNA:   3'- -CUCGUAGaaGUGCUacaGGCGUCCGu -5'
33103 5' -53.8 NC_007497.1 + 4597 1.09 0.000953
Target:  5'- cGAGCAUCUUCACGAUGUCCGCAGGCAu -3'
miRNA:   3'- -CUCGUAGAAGUGCUACAGGCGUCCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.