miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
33104 3' -50.9 NC_007497.1 + 43512 0.66 0.820539
Target:  5'- aAGuCGGCAUUUGAugcAGUGCggUUCGCGc -3'
miRNA:   3'- -UC-GUCGUAGACUu--UCACGa-AGGUGCa -5'
33104 3' -50.9 NC_007497.1 + 1423 0.69 0.690239
Target:  5'- cGCAGCGUCcGAuuGcGCggCCACGg -3'
miRNA:   3'- uCGUCGUAGaCUuuCaCGaaGGUGCa -5'
33104 3' -50.9 NC_007497.1 + 41776 0.7 0.596769
Target:  5'- aGGCGGCAauUCUGAAAGcGCU-CgGCGa -3'
miRNA:   3'- -UCGUCGU--AGACUUUCaCGAaGgUGCa -5'
33104 3' -50.9 NC_007497.1 + 5936 1.09 0.001817
Target:  5'- gAGCAGCAUCUGAAAGUGCUUCCACGUg -3'
miRNA:   3'- -UCGUCGUAGACUUUCACGAAGGUGCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.