Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33107 | 5' | -54 | NC_007497.1 | + | 7212 | 1.1 | 0.000995 |
Target: 5'- gUGGUACUCCCGUCCGCAUUGCGUUCGa -3' miRNA: 3'- -ACCAUGAGGGCAGGCGUAACGCAAGC- -5' |
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33107 | 5' | -54 | NC_007497.1 | + | 25414 | 0.68 | 0.602386 |
Target: 5'- ---cGCUUCCGUCCGCA--GCGUUg- -3' miRNA: 3'- accaUGAGGGCAGGCGUaaCGCAAgc -5' |
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33107 | 5' | -54 | NC_007497.1 | + | 4217 | 0.67 | 0.658428 |
Target: 5'- gGGUAUUCCCGguccaugcgCCGUgaUGCGcUCa -3' miRNA: 3'- aCCAUGAGGGCa--------GGCGuaACGCaAGc -5' |
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33107 | 5' | -54 | NC_007497.1 | + | 35474 | 0.66 | 0.7247 |
Target: 5'- cGGUACUagcgCCGgcauUCCGCAgcGCG-UCGa -3' miRNA: 3'- aCCAUGAg---GGC----AGGCGUaaCGCaAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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