Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33108 | 5' | -54 | NC_007497.1 | + | 1643 | 0.66 | 0.658556 |
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Target: 5'- aAGAG-GUGGGCGGAacuggcucGCAugGGCg--CACg -3' miRNA: 3'- -UCUCgCACCCGUCU--------CGU--UCGaaaGUG- -5' |
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| 33108 | 5' | -54 | NC_007497.1 | + | 6436 | 0.67 | 0.636715 |
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Target: 5'- uGGGCGcgaucuacugcauugGGGCgAGGGUucccGGCUUUCGCa -3' miRNA: 3'- uCUCGCa--------------CCCG-UCUCGu---UCGAAAGUG- -5' |
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| 33108 | 5' | -54 | NC_007497.1 | + | 43141 | 0.69 | 0.489388 |
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Target: 5'- cGAGCGcucGGGCAGuuucucGCGAGCgaugCACu -3' miRNA: 3'- uCUCGCa--CCCGUCu-----CGUUCGaaa-GUG- -5' |
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| 33108 | 5' | -54 | NC_007497.1 | + | 38661 | 0.7 | 0.417234 |
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Target: 5'- uGGAgGCGUGGGCGc-GCGAGCgcggUUACg -3' miRNA: 3'- -UCU-CGCACCCGUcuCGUUCGaa--AGUG- -5' |
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| 33108 | 5' | -54 | NC_007497.1 | + | 17376 | 0.71 | 0.397835 |
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Target: 5'- cGGGGCGaugagcGGGCGaAGCGcaaAGCUUUCACc -3' miRNA: 3'- -UCUCGCa-----CCCGUcUCGU---UCGAAAGUG- -5' |
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| 33108 | 5' | -54 | NC_007497.1 | + | 8424 | 1.11 | 0.00059 |
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Target: 5'- gAGAGCGUGGGCAGAGCAAGCUUUCACg -3' miRNA: 3'- -UCUCGCACCCGUCUCGUUCGAAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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