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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33109 | 5' | -51.2 | NC_007497.1 | + | 9396 | 0.66 | 0.87721 |
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Target: 5'- -aCCGAGAUCGucgcguacguuGGuCGAGCgAACGAa- -3' miRNA: 3'- aaGGCUCUAGU-----------UC-GCUCGaUUGCUcc -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 41661 | 0.66 | 0.87721 |
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Target: 5'- gUCCGAGcgCGAGacaGAGCcgcuCGAGu -3' miRNA: 3'- aAGGCUCuaGUUCg--CUCGauu-GCUCc -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 6228 | 0.69 | 0.696498 |
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Target: 5'- -gCUGAGAUCGAGCGAuCUGAcCGAa- -3' miRNA: 3'- aaGGCUCUAGUUCGCUcGAUU-GCUcc -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 43880 | 0.7 | 0.616062 |
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Target: 5'- cUUCGGGGUgAAGCGcagccuGGCUGACGuGGc -3' miRNA: 3'- aAGGCUCUAgUUCGC------UCGAUUGCuCC- -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 7513 | 0.7 | 0.614909 |
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Target: 5'- gUCCGAGAUCGAcgcaaccacgcucGCGAGCgaa-GAGa -3' miRNA: 3'- aAGGCUCUAGUU-------------CGCUCGauugCUCc -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 10348 | 0.75 | 0.372008 |
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Target: 5'- gUUCCGGGAUCAuGCGAGCagcGCGAc- -3' miRNA: 3'- -AAGGCUCUAGUuCGCUCGau-UGCUcc -5' |
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| 33109 | 5' | -51.2 | NC_007497.1 | + | 8673 | 1.08 | 0.002315 |
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Target: 5'- uUUCCGAGAUCAAGCGAGCUAACGAGGc -3' miRNA: 3'- -AAGGCUCUAGUUCGCUCGAUUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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