Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33113 | 5' | -50.7 | NC_007497.1 | + | 10149 | 0.69 | 0.76115 |
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Target: 5'- cCGAacGAGGCgccaccgCGGCGAcCgccgGCUUgGCCg -3' miRNA: 3'- -GCU--UUCCGa------GCCGUUaGa---CGAAgUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 27091 | 0.69 | 0.738301 |
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Target: 5'- uGAAGGGCgauuccgUCGcGCGAUgaGCUcgCGCCg -3' miRNA: 3'- gCUUUCCG-------AGC-CGUUAgaCGAa-GUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 30080 | 0.7 | 0.693443 |
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Target: 5'- uCGAGcucGGGCauggUGGUGAUCucguucaUGCUUCACCu -3' miRNA: 3'- -GCUU---UCCGa---GCCGUUAG-------ACGAAGUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 16978 | 0.71 | 0.6256 |
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Target: 5'- uGAAAGGCgcaUGGCAAUCgaUGC--CGCCg -3' miRNA: 3'- gCUUUCCGa--GCCGUUAG--ACGaaGUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 13448 | 0.72 | 0.579574 |
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Target: 5'- aGAGcaacGGGCUCGacgauGCGAUCUggacGCUUCGCUg -3' miRNA: 3'- gCUU----UCCGAGC-----CGUUAGA----CGAAGUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 7882 | 0.72 | 0.568163 |
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Target: 5'- ----cGGCaacUCGGCGagGUCUGCUgcacUCACCg -3' miRNA: 3'- gcuuuCCG---AGCCGU--UAGACGA----AGUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 29178 | 0.72 | 0.568162 |
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Target: 5'- uCGAAAcGCUCGGCucgCUGUUccagCACCa -3' miRNA: 3'- -GCUUUcCGAGCCGuuaGACGAa---GUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 33071 | 0.74 | 0.45852 |
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Target: 5'- uGAAAGcGCaUCGGCuAUCgaUGCUUCACUc -3' miRNA: 3'- gCUUUC-CG-AGCCGuUAG--ACGAAGUGG- -5' |
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| 33113 | 5' | -50.7 | NC_007497.1 | + | 11071 | 1.14 | 0.001046 |
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Target: 5'- gCGAAAGGCUCGGCAAUCUGCUUCACCg -3' miRNA: 3'- -GCUUUCCGAGCCGUUAGACGAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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