Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33114 | 3' | -51.2 | NC_007497.1 | + | 27104 | 0.71 | 0.577559 |
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Target: 5'- cAGcGGAGCGgauaCGGccGCCGAUGCGCc -3' miRNA: 3'- cUCaCUUCGUa---GCUauCGGCUACGCG- -5' |
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| 33114 | 3' | -51.2 | NC_007497.1 | + | 6886 | 0.71 | 0.566148 |
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Target: 5'- ---cGAcGCGUCGAUcgcucaGGCCGAggGCGCa -3' miRNA: 3'- cucaCUuCGUAGCUA------UCGGCUa-CGCG- -5' |
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| 33114 | 3' | -51.2 | NC_007497.1 | + | 16112 | 0.71 | 0.552534 |
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Target: 5'- -cGUGAGGCuUCGAaguggaugcggcgcaUGGCCGAgcauggaaacggcguUGCGCu -3' miRNA: 3'- cuCACUUCGuAGCU---------------AUCGGCU---------------ACGCG- -5' |
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| 33114 | 3' | -51.2 | NC_007497.1 | + | 34038 | 0.73 | 0.467075 |
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Target: 5'- --cUGccGUAUCGAUuGCCGAUGCGUc -3' miRNA: 3'- cucACuuCGUAGCUAuCGGCUACGCG- -5' |
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| 33114 | 3' | -51.2 | NC_007497.1 | + | 35177 | 0.83 | 0.10864 |
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Target: 5'- -uGUGAAGCAUCGAUAcuauuGCCGAagcaguuUGCGCg -3' miRNA: 3'- cuCACUUCGUAGCUAU-----CGGCU-------ACGCG- -5' |
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| 33114 | 3' | -51.2 | NC_007497.1 | + | 11711 | 1.13 | 0.001068 |
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Target: 5'- cGAGUGAAGCAUCGAUAGCCGAUGCGCu -3' miRNA: 3'- -CUCACUUCGUAGCUAUCGGCUACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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