Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33124 | 5' | -53.1 | NC_007497.1 | + | 3696 | 0.66 | 0.772951 |
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Target: 5'- aGAuGGUCGGCGuaGCGCU-GCUCGGu -3' miRNA: 3'- cCUuCCAGCUGCuaCGUGAgCGAGUU- -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 39721 | 0.66 | 0.762453 |
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Target: 5'- cGAAGGcguUgGGCGAauggGCGCUCGCUgAGc -3' miRNA: 3'- cCUUCC---AgCUGCUa---CGUGAGCGAgUU- -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 14066 | 0.66 | 0.741039 |
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Target: 5'- cGAAGGUCGAgCGAaGCAUccUGCUCu- -3' miRNA: 3'- cCUUCCAGCU-GCUaCGUGa-GCGAGuu -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 6427 | 0.66 | 0.741039 |
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Target: 5'- cGGGAGGacaUCGACagcgaGAUGCGCauUCGCUa-- -3' miRNA: 3'- -CCUUCC---AGCUG-----CUACGUG--AGCGAguu -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 13537 | 0.68 | 0.640302 |
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Target: 5'- cGGAAGGUCGAacaccUGAUGCAagaccagCGCagCAAg -3' miRNA: 3'- -CCUUCCAGCU-----GCUACGUga-----GCGa-GUU- -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 14341 | 0.73 | 0.366903 |
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Target: 5'- aGGAAaGUCGACGAaGUGCUCGC-CGAa -3' miRNA: 3'- -CCUUcCAGCUGCUaCGUGAGCGaGUU- -5' |
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| 33124 | 5' | -53.1 | NC_007497.1 | + | 16703 | 1.08 | 0.001354 |
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Target: 5'- aGGAAGGUCGACGAUGCACUCGCUCAAc -3' miRNA: 3'- -CCUUCCAGCUGCUACGUGAGCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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