Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33125 | 5' | -48.7 | NC_007497.1 | + | 5098 | 0.66 | 0.967249 |
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Target: 5'- aCCcAGUCGUcGGGuAAGCGCGuuccgCGc -3' miRNA: 3'- -GGcUUAGCAaCCC-UUCGCGCuuaa-GC- -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 10338 | 0.66 | 0.967249 |
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Target: 5'- aCUGGcUUGUguuccGGGAucaugcgagcAGCGCGAcgUCGa -3' miRNA: 3'- -GGCUuAGCAa----CCCU----------UCGCGCUuaAGC- -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 4566 | 0.67 | 0.944784 |
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Target: 5'- uCCGAcgCGUUGGcGAaggucgacaaggucAGCGCuGAucUCGa -3' miRNA: 3'- -GGCUuaGCAACC-CU--------------UCGCG-CUuaAGC- -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 12621 | 0.67 | 0.94121 |
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Target: 5'- gCCGAcgCGUcgcagaugaUGGcGAGCGcCGAGUUUGc -3' miRNA: 3'- -GGCUuaGCA---------ACCcUUCGC-GCUUAAGC- -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 11020 | 0.68 | 0.89736 |
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Target: 5'- gCCGggUUGauugcGGGgcGCGCGGAUg-- -3' miRNA: 3'- -GGCuuAGCaa---CCCuuCGCGCUUAagc -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 12891 | 0.68 | 0.89736 |
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Target: 5'- uUCGAuUCG-UGGGgcGCGCGGAc--- -3' miRNA: 3'- -GGCUuAGCaACCCuuCGCGCUUaagc -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 41683 | 0.74 | 0.624058 |
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Target: 5'- cUCGAGUC---GGGAAGUGgGGAUUCGa -3' miRNA: 3'- -GGCUUAGcaaCCCUUCGCgCUUAAGC- -5' |
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| 33125 | 5' | -48.7 | NC_007497.1 | + | 17746 | 1.14 | 0.002334 |
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Target: 5'- aCCGAAUCGUUGGGAAGCGCGAAUUCGg -3' miRNA: 3'- -GGCUUAGCAACCCUUCGCGCUUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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