miRNA: 3'- -GCauau-UCGCCCUA-CGCCuCGCCaua -5'
miRNA: 3'- gCAUaUUCGCCcuaCGCC-UCGCCAUa -5'
miRNA: 3'- gCAuaU-UCGC-CCU---ACGCCUCGCCAUa -5'
miRNA: 3'- -GCAUAUUCGCCCUACGCCUCGCCAUA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33126 | 5' | -53.7 | NC_007497.1 | + | 23250 | 0.66 | 0.698788 |
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Target: 5'- gCGgaaguGGCGGGAUgGCGG-GCGGc-- -3' miRNA: 3'- -GCauau-UCGCCCUA-CGCCuCGCCaua -5' |
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| 33126 | 5' | -53.7 | NC_007497.1 | + | 16234 | 0.67 | 0.653633 |
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Target: 5'- gGUGgAGGCGGcg-GCGGcGGCGGUGg -3' miRNA: 3'- gCAUaUUCGCCcuaCGCC-UCGCCAUa -5' |
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| 33126 | 5' | -53.7 | NC_007497.1 | + | 2650 | 0.71 | 0.42515 |
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Target: 5'- aGUcgAuGGCGcGGAucgUGUGGAGCGGUAa -3' miRNA: 3'- gCAuaU-UCGC-CCU---ACGCCUCGCCAUa -5' |
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| 33126 | 5' | -53.7 | NC_007497.1 | + | 19038 | 1.05 | 0.001807 |
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Target: 5'- gCGUAUAAGCGGGAUGCGGAGCGGUAUc -3' miRNA: 3'- -GCAUAUUCGCCCUACGCCUCGCCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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