Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33130 | 3' | -49.8 | NC_007497.1 | + | 17801 | 0.66 | 0.94121 |
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Target: 5'- cGcCGuGAAGUC-GGUGGACGGcgacaCGCu -3' miRNA: 3'- aCuGC-CUUCAGaUCACCUGCUua---GCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 12272 | 0.66 | 0.935296 |
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Target: 5'- cGGCGGA---CUGGcGGGCGGccugucggauggcGUCGCg -3' miRNA: 3'- aCUGCCUucaGAUCaCCUGCU-------------UAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 14961 | 0.66 | 0.930187 |
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Target: 5'- cGGCGGGugAGUgUAuuugccgGGGCGAaauGUCGCg -3' miRNA: 3'- aCUGCCU--UCAgAUca-----CCUGCU---UAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 16242 | 0.68 | 0.848468 |
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Target: 5'- cGGCGGcGG-CggcGGUGGA-GAGUCGCc -3' miRNA: 3'- aCUGCCuUCaGa--UCACCUgCUUAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 2403 | 0.68 | 0.839402 |
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Target: 5'- -cGCGGcGAGcugaaUGGUGGcACGggUCGCg -3' miRNA: 3'- acUGCC-UUCag---AUCACC-UGCuuAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 13513 | 0.69 | 0.810816 |
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Target: 5'- uUGGCGGAaaccucacGGUC-GGUGGGCGuGUCcuGCu -3' miRNA: 3'- -ACUGCCU--------UCAGaUCACCUGCuUAG--CG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 12550 | 0.71 | 0.72649 |
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Target: 5'- cUGGCGGGcAGUUcGGUGG-CGAuagCGCg -3' miRNA: 3'- -ACUGCCU-UCAGaUCACCuGCUua-GCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 2642 | 0.72 | 0.635565 |
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Target: 5'- cGACGGcGAGUCga-UGGcGCGGAUCGUg -3' miRNA: 3'- aCUGCC-UUCAGaucACC-UGCUUAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 22652 | 0.74 | 0.544307 |
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Target: 5'- gUGAa-GAAGUaacgCUAgGUGGACGAGUCGCc -3' miRNA: 3'- -ACUgcCUUCA----GAU-CACCUGCUUAGCG- -5' |
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| 33130 | 3' | -49.8 | NC_007497.1 | + | 22092 | 1.13 | 0.001762 |
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Target: 5'- gUGACGGAAGUCUAGUGGACGAAUCGCu -3' miRNA: 3'- -ACUGCCUUCAGAUCACCUGCUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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