Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33130 | 5' | -54.2 | NC_007497.1 | + | 9964 | 0.66 | 0.753128 |
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Target: 5'- aGGGCGAgCUCGagcgUCCGaggcgucgCUGGCGUUGg- -3' miRNA: 3'- -CCCGCU-GAGC----AGGUg-------GAUCGCAAUga -5' |
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| 33130 | 5' | -54.2 | NC_007497.1 | + | 9524 | 0.66 | 0.753128 |
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Target: 5'- cGGGCGGCUCGUCacguauUugCUgcaaAGUGguauUGCUg -3' miRNA: 3'- -CCCGCUGAGCAG------GugGA----UCGCa---AUGA- -5' |
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| 33130 | 5' | -54.2 | NC_007497.1 | + | 27071 | 0.66 | 0.70695 |
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Target: 5'- cGGGCGcCUUGUCCaacacgacaaccgcGCCggcAGCGgaGCg -3' miRNA: 3'- -CCCGCuGAGCAGG--------------UGGa--UCGCaaUGa -5' |
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| 33130 | 5' | -54.2 | NC_007497.1 | + | 43611 | 0.7 | 0.500951 |
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Target: 5'- cGGCGGCUCGUC-GCUUuGCGUUGu- -3' miRNA: 3'- cCCGCUGAGCAGgUGGAuCGCAAUga -5' |
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| 33130 | 5' | -54.2 | NC_007497.1 | + | 28012 | 0.78 | 0.157673 |
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Target: 5'- cGGGCuGCUCGUCCGCCggcGCGggUGCg -3' miRNA: 3'- -CCCGcUGAGCAGGUGGau-CGCa-AUGa -5' |
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| 33130 | 5' | -54.2 | NC_007497.1 | + | 22128 | 1.1 | 0.000975 |
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Target: 5'- aGGGCGACUCGUCCACCUAGCGUUACUu -3' miRNA: 3'- -CCCGCUGAGCAGGUGGAUCGCAAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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