Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33131 | 5' | -52.1 | NC_007497.1 | + | 23386 | 0.66 | 0.823249 |
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Target: 5'- --aUUGGGCCgcggUGCGgCGUUGUUCUuCGg -3' miRNA: 3'- cucAGCCCGG----AUGCgGUAAUAAGA-GC- -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 7558 | 0.66 | 0.813616 |
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Target: 5'- cGAGcUGGGCCUGCcggaucCCGgcgaAUUCUCGg -3' miRNA: 3'- -CUCaGCCCGGAUGc-----GGUaa--UAAGAGC- -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 21972 | 0.67 | 0.783531 |
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Target: 5'- cGAGuUCGGcGUCUGCGCCcggUGUUC-Ca -3' miRNA: 3'- -CUC-AGCC-CGGAUGCGGua-AUAAGaGc -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 36036 | 0.68 | 0.730163 |
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Target: 5'- -cGUCGGcacagcuuGCCUGCGCCggUAUcgCUCu -3' miRNA: 3'- cuCAGCC--------CGGAUGCGGuaAUAa-GAGc -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 26288 | 0.71 | 0.52689 |
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Target: 5'- -cGUCGGGCCg--GCCA--GUUCUCu -3' miRNA: 3'- cuCAGCCCGGaugCGGUaaUAAGAGc -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 32040 | 0.72 | 0.483607 |
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Target: 5'- -uGUCGGGCgCUACcCCGU--UUCUCGg -3' miRNA: 3'- cuCAGCCCG-GAUGcGGUAauAAGAGC- -5' |
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| 33131 | 5' | -52.1 | NC_007497.1 | + | 22667 | 1.09 | 0.001685 |
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Target: 5'- cGAGUCGGGCCUACGCCAUUAUUCUCGc -3' miRNA: 3'- -CUCAGCCCGGAUGCGGUAAUAAGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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