Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33133 | 5' | -53.2 | NC_007497.1 | + | 23405 | 0.66 | 0.741039 |
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Target: 5'- uUgUUCUUCGGCGCGcgCAG-CAUCu- -3' miRNA: 3'- cAgAAGAAGCCGUGCa-GUCgGUAGca -5' |
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| 33133 | 5' | -53.2 | NC_007497.1 | + | 38936 | 0.67 | 0.719155 |
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Target: 5'- -aCUUCUUCGGUGCGgUCGGCU-UCu- -3' miRNA: 3'- caGAAGAAGCCGUGC-AGUCGGuAGca -5' |
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| 33133 | 5' | -53.2 | NC_007497.1 | + | 22568 | 0.68 | 0.605016 |
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Target: 5'- uUCUUCUcCGGCGuaucgauauauccCGUCAuGUCGUCGUa -3' miRNA: 3'- cAGAAGAaGCCGU-------------GCAGU-CGGUAGCA- -5' |
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| 33133 | 5' | -53.2 | NC_007497.1 | + | 3899 | 0.69 | 0.572217 |
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Target: 5'- cGUCaUCUUCGGuCGCG--AGUCGUCGUg -3' miRNA: 3'- -CAGaAGAAGCC-GUGCagUCGGUAGCA- -5' |
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| 33133 | 5' | -53.2 | NC_007497.1 | + | 36095 | 0.69 | 0.560997 |
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Target: 5'- -aCUUCUUCGGagACGcCGGCC-UCGUu -3' miRNA: 3'- caGAAGAAGCCg-UGCaGUCGGuAGCA- -5' |
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| 33133 | 5' | -53.2 | NC_007497.1 | + | 24039 | 1.08 | 0.001313 |
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Target: 5'- cGUCUUCUUCGGCACGUCAGCCAUCGUg -3' miRNA: 3'- -CAGAAGAAGCCGUGCAGUCGGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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