Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33137 | 5' | -50.7 | NC_007497.1 | + | 14046 | 0.66 | 0.856019 |
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Target: 5'- -cGAACGUGAAUGUCccAUGUUgGGg-- -3' miRNA: 3'- aaCUUGCACUUGCAG--UGCAAgCCgua -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 32962 | 0.67 | 0.837787 |
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Target: 5'- ----uCGUGAAauCGUCACG-UCGGCGa -3' miRNA: 3'- aacuuGCACUU--GCAGUGCaAGCCGUa -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 28522 | 0.67 | 0.798496 |
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Target: 5'- gUUGAACG-GAGCG-CGcCGgccgUCGGCGUa -3' miRNA: 3'- -AACUUGCaCUUGCaGU-GCa---AGCCGUA- -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 7016 | 0.68 | 0.788153 |
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Target: 5'- -aGGcuACGUGucCGUCGCGagUUCGGCGg -3' miRNA: 3'- aaCU--UGCACuuGCAGUGC--AAGCCGUa -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 13176 | 0.68 | 0.756079 |
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Target: 5'- aUGAACGcGAACGUCgAUGUUCucaGCAUg -3' miRNA: 3'- aACUUGCaCUUGCAG-UGCAAGc--CGUA- -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 18296 | 0.69 | 0.733962 |
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Target: 5'- aUGAACGUG-ACGUCGCugcCGGCc- -3' miRNA: 3'- aACUUGCACuUGCAGUGcaaGCCGua -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 25655 | 0.69 | 0.722724 |
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Target: 5'- gUUGAGCGUcaauGGCGUCGuccaguUGUUCGGCAc -3' miRNA: 3'- -AACUUGCAc---UUGCAGU------GCAAGCCGUa -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 19950 | 0.74 | 0.419789 |
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Target: 5'- -cGAACGUG-ACGUCACGUUCaauGCGa -3' miRNA: 3'- aaCUUGCACuUGCAGUGCAAGc--CGUa -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 3837 | 0.74 | 0.419789 |
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Target: 5'- gUGAACGUGAugGggcUUGCGgcgUCGGCAg -3' miRNA: 3'- aACUUGCACUugC---AGUGCa--AGCCGUa -5' |
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| 33137 | 5' | -50.7 | NC_007497.1 | + | 24842 | 1.03 | 0.004947 |
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Target: 5'- aUUGAACGUGAACGUCACGUUCGGCAUc -3' miRNA: 3'- -AACUUGCACUUGCAGUGCAAGCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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