Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33140 | 3' | -56.3 | NC_007497.1 | + | 1022 | 0.72 | 0.290396 |
Target: 5'- gGUUGcGGCCGACACUGACACgguaucggGCa- -3' miRNA: 3'- gCGAC-CCGGCUGUGGUUGUGaa------CGcu -5' |
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33140 | 3' | -56.3 | NC_007497.1 | + | 14334 | 0.73 | 0.248213 |
Target: 5'- aCGCcaGGcaGCCGGCGCCGACACUUacugucgGCGAc -3' miRNA: 3'- -GCGa-CC--CGGCUGUGGUUGUGAA-------CGCU- -5' |
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33140 | 3' | -56.3 | NC_007497.1 | + | 42954 | 0.74 | 0.195896 |
Target: 5'- gGCcGGGUCGACGCCGGC-CUUGUa- -3' miRNA: 3'- gCGaCCCGGCUGUGGUUGuGAACGcu -5' |
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33140 | 3' | -56.3 | NC_007497.1 | + | 25631 | 1.09 | 0.000602 |
Target: 5'- cCGCUGGGCCGACACCAACACUUGCGAu -3' miRNA: 3'- -GCGACCCGGCUGUGGUUGUGAACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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