Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33142 | 5' | -53.7 | NC_007497.1 | + | 33099 | 0.66 | 0.770688 |
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Target: 5'- -gCCAgcUCGGCGUCGaucUgCGCGCUGc -3' miRNA: 3'- ggGGU--AGUCGUAGCaa-AgGCGCGACu -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 20594 | 0.66 | 0.770688 |
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Target: 5'- -gCCGU-GGCGUCGUcgCUGaCGCUGAc -3' miRNA: 3'- ggGGUAgUCGUAGCAaaGGC-GCGACU- -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 33817 | 0.66 | 0.749748 |
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Target: 5'- cCCCCuucGUCuucGCcUCGUaUUCCGCGCg-- -3' miRNA: 3'- -GGGG---UAGu--CGuAGCA-AAGGCGCGacu -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 42035 | 0.67 | 0.677634 |
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Target: 5'- cCCCgGUCGGCGUCGUguagucguagacgaUCUGCcacGUUGAc -3' miRNA: 3'- -GGGgUAGUCGUAGCAa-------------AGGCG---CGACU- -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 24950 | 0.69 | 0.560057 |
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Target: 5'- uUCCAUCAgGCGUCGUaaugcgaaggcccUgaaaaugcaCCGCGCUGAu -3' miRNA: 3'- gGGGUAGU-CGUAGCA-------------Aa--------GGCGCGACU- -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 23098 | 0.7 | 0.517504 |
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Target: 5'- uCCCUAUCGuuagcGCGUCGUagUCGCGCUc- -3' miRNA: 3'- -GGGGUAGU-----CGUAGCAaaGGCGCGAcu -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 24882 | 0.7 | 0.485669 |
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Target: 5'- uCCCUAUUAGCGUCGUUguacgCCaaGUGCaGAu -3' miRNA: 3'- -GGGGUAGUCGUAGCAAa----GG--CGCGaCU- -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 40285 | 0.74 | 0.302779 |
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Target: 5'- aCCUAcaaCAGCGUCGUUUUcgacgaCGCGCUGAu -3' miRNA: 3'- gGGGUa--GUCGUAGCAAAG------GCGCGACU- -5' |
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| 33142 | 5' | -53.7 | NC_007497.1 | + | 26466 | 1.1 | 0.000926 |
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Target: 5'- uCCCCAUCAGCAUCGUUUCCGCGCUGAc -3' miRNA: 3'- -GGGGUAGUCGUAGCAAAGGCGCGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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