Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33143 | 5' | -55.6 | NC_007497.1 | + | 12107 | 0.66 | 0.620439 |
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Target: 5'- cGCAGCGACCgGGaaGCUGgauuUCCg- -3' miRNA: 3'- -UGUCGCUGGgUCagCGGCaau-AGGaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 3454 | 0.66 | 0.609168 |
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Target: 5'- cGCuGaCGAUCCGGUCGCCGaucagcUCCg- -3' miRNA: 3'- -UGuC-GCUGGGUCAGCGGCaau---AGGaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 44643 | 0.66 | 0.597917 |
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Target: 5'- gGC-GCGACCCGGcCGCCGaugcUGUCg-- -3' miRNA: 3'- -UGuCGCUGGGUCaGCGGCa---AUAGgaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 21867 | 0.66 | 0.586698 |
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Target: 5'- gACAGCGGCCCuGacUCGUCGggGcCCg- -3' miRNA: 3'- -UGUCGCUGGGuC--AGCGGCaaUaGGaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 26908 | 0.67 | 0.520519 |
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Target: 5'- gGCGGCGGgcaacaacuUCCAGUCGCC---AUCCUc -3' miRNA: 3'- -UGUCGCU---------GGGUCAGCGGcaaUAGGAa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 44242 | 0.68 | 0.477007 |
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Target: 5'- uCAGCcgccuuuCCCAGUCgggacauGCCGUUGUCCa- -3' miRNA: 3'- uGUCGcu-----GGGUCAG-------CGGCAAUAGGaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 7038 | 0.69 | 0.408177 |
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Target: 5'- uCGGCGGCCCAaUCGCCGUacuacguggUGUCg-- -3' miRNA: 3'- uGUCGCUGGGUcAGCGGCA---------AUAGgaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 18194 | 0.71 | 0.320221 |
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Target: 5'- aACGGCGACUgGGUCGCUGUUGc---- -3' miRNA: 3'- -UGUCGCUGGgUCAGCGGCAAUaggaa -5' |
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| 33143 | 5' | -55.6 | NC_007497.1 | + | 26599 | 1.04 | 0.001324 |
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Target: 5'- aACAGCGACCCAGUCGCCGUUAUCCUUc -3' miRNA: 3'- -UGUCGCUGGGUCAGCGGCAAUAGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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